Genomic characterization of XDR Klebsiella pneumoniae ST147 co-resistant to carbapenem and colistin - The first report in India

被引：19

作者：

Dey, Suchanda ^{[1
]}

Gaur, Mahendra ^{[1
]}

Sahoo, Rajesh Kumar ^{[1
]}

Das, Aradhana ^{[1
]}

Jain, Bhawana ^{[2
]}

Pati, Sanghamitra ^{[3
]}

Subudhi, Enketeswara ^{[1
]}

机构：

[1] Siksha O Anusandhan Deemed Be Univ, Sch Pharmaceut Sci, Ctr Biotechnol, Bhubaneswar 751003, Odisha, India

[2] Vivekananda Polyclin & Inst Med Sci, Dept Microbiol, Lucknow 226007, Uttar Pradesh, India

[3] Indian Council Med Res ICMR, Reg Med Res Ctr, Bhubaneswar 751023, Odisha, India

来源：

JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE | 2020年 / 22卷

关键词：

Colistin; Efflux pump; Klebsiella pneumoniae; Co-resistance; Whole-genome sequencing;

D O I：

10.1016/j.jgar.2020.05.005

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: The emergence and outbreak of colistin-resistant CRKP (carbapenem-resistant Klebsiella pneumoniae) have been the major global public threat in recent years. Present study emphasized the genome-wide distribution, characterization of drug resistance virulence genes in an extremely drug-resistant (XDR) Klebsiella pneumoniae strain isolated from a patient with drug-induced hepatitis, hospitalized in a tertiary care facility in India. Methods: The total genomic DNA was sequenced using the Illumina Hiseq platform. De novo assembly of reads was done using CLC genomics workbench. Genome annotation was performed using PROKKA. Sequence typing (ST), virulence-related genes and antimicrobial resistance genes were predicted from genome sequences. Phenotypic evaluation of efflux pump function was done in presence of colistin and efflux pump inhibitor (EPI). Result: Antibiogram analysis confirmed the isolate to be XDR. The number of contigs in assembly file was found to be 867 with a total of 6,060,836 bases and a total of 5547 coding sequences. The isolate exhibited high resistance to colistin due to mutations in two-component systems and predicted to be efflux mediated. The sequence typing of Klebsiella pneumoniae SDL79 is assigned to ST147. Conclusion: This is the first whole genome analysis of XDR Klebsiella pneumoniae ST147 from a hospital conferring co-resistance to last resort drugs. However, the detailed molecular mechanism behind the drug resistance will be carried out in our future endeavors. (C) 2020 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd.

引用

页码：54 / 56

页数：3

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