Identification of tetranectin as adipogenic serum protein

被引：10

作者：

Park, Jihyun ^{[1
]}

Park, Jeongho ^{[1
]}

Jeong, Jinju ^{[1
]}

Cho, Kyung-Hyun ^{[1
]}

Choi, Inho ^{[1
]}

Kim, Jihoe ^{[1
]}

机构：

[1] Yeungnam Univ, Sch Biotechnol, Gyongsan 712749, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 460卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

Serum; Adipocyte; Differentiation; Tetranectin; Proteome; ADIPOSE CONVERSION; PLASMA; BINDING; MODELS;

D O I：

10.1016/j.bbrc.2015.03.073

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fetal bovine serum (FBS) is an essential culture supplement for adipocyte differentiation of various adipogenic precursor cells. Adipocyte differentiation greatly varies depending on the type of serum in the differentiation medium. In this study, we found that FBS supported adipocyte differentiation of 3T3-L1 cells to a significantly higher extent than other types of bovine serum such as adult bovine serum (ABS). This differential adipogenic effect of bovine serum was shown to be due to the protein contents of bovine sera, indicating the presence of an adipogenic protein(s) in FBS. Serum proteome analysis identified tetranectin as an adipogenic protein. The adipogenic effect of tetranectin was confirmed by supplementation of FBS-containing differentiation medium with anti-tetranectin antibody, which suppressed adipocyte differentiation of 3T3-L1 cells. These results demonstrate that tetranectin is an adipogenic serum protein mediating the adipogenic effect of FBS. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：583 / 588

页数：6

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