Two novel tubuliform silk gene sequences from Araneus ventricosus provide evidence for multiple loci in genome

被引：11
作者：
Wen, Rui [1 ]
Wang, Kangkang [1 ]
Meng, Qing [1 ]
机构：
[1] Donghua Univ, Inst Biol Sci & Biotechnol, Shanghai, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 160卷
基金：
中国国家自然科学基金;
关键词：
Concerted evolution; Multiple loci; TuSp1; EGG CASE SILK; MECHANICAL-PROPERTIES; SPIDER SILKS; WASP SPIDER; EVOLUTION; PROTEINS; CONSERVATION; FLAGELLIFORM; DIVERSITY; ALIGNMENT;
D O I：
10.1016/j.ijbiomac.2020.05.141
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Spiders produce a diversity of silk fibers from multiple morphologically distinct silk glands for specific tasks, and each silk type primarily composed of one or more particular silk proteins encoded by silk gene family members believed to generated by duplication and divergence of ancient silk genes. Egg case silks spun from tubuliform glands are used to construct the tough outer structure of egg cases, are important for their reproduction. Here we present two novel complete TuSp1 sequences from orb weaving spider Araneus ventricosus. Alignment of the two spidroin iterated repeats showed both extreme intragenic homogenization. The pairwise Ka/Ks analysis revealed the terminal and repetitive regions for three TuSp1 loci including the reported TuSp1 gene are all under purifying selection. Phylogenetic analysis showed the two new TuSp1 variants could derive from recent duplication events. (C) 2020 Elsevier B.V. All rights reserved.
引用
收藏
页码：806 / 813
页数：8
相关论文
共 46 条
[1]   Nephila clavipes Flagelliform Silk-Like GGX Motifs Contribute to Extensibility and Spacer Motifs Contribute to Strength in Synthetic Spider Silk Fibers [J].
Adrianos, Sherry L. ;
Teule, Florence ;
Hinman, Michael B. ;
Jones, Justin A. ;
Weber, Warner S. ;
Yarger, Jeffery L. ;
Lewis, Randolph V. .
BIOMACROMOLECULES, 2013, 14 (06) :1751-1760
[2]   Bioprospecting Finds the Toughest Biological Material: Extraordinary Silk from a Giant Riverine Orb Spider [J].
Agnarsson, Ingi ;
Kuntner, Matjaz ;
Blackledge, Todd A. .
PLOS ONE, 2010, 5 (09) :1-8
[3]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[4]  
[Anonymous], 2011, Biology of spiders
[5]   SignalP 5.0 improves signal peptide predictions using deep neural networks [J].
Armenteros, Jose Juan Almagro ;
Tsirigos, Konstantinos D. ;
Sonderby, Casper Kaae ;
Petersen, Thomas Nordahl ;
Winther, Ole ;
Brunak, Soren ;
von Heijne, Gunnar ;
Nielsen, Henrik .
NATURE BIOTECHNOLOGY, 2019, 37 (04) :420-+
[6]   Self-assembly of spider silk proteins is controlled by a pH-sensitive relay [J].
Askarieh, Glareh ;
Hedhammar, My ;
Nordling, Kerstin ;
Saenz, Alejandra ;
Casals, Cristina ;
Rising, Anna ;
Johansson, Jan ;
Knight, Stefan D. .
NATURE, 2010, 465 (7295) :236-U125
[7]   Multiple recombining loci encode MaSp1, the primary constituent of dragline silk, in widow spiders (Latrodectus: Theridiidae) [J].
Ayoub, Nadia A. ;
Hayashi, Cheryl Y. .
MOLECULAR BIOLOGY AND EVOLUTION, 2008, 25 (02) :277-286
[8]   Blueprint for a High-Performance Biomaterial: Full-Length Spider Dragline Silk Genes [J].
Ayoub, Nadia A. ;
Garb, Jessica E. ;
Tinghitella, Robin M. ;
Collin, Matthew A. ;
Hayashi, Cheryl Y. .
PLOS ONE, 2007, 2 (06)
[9]   Ancient Properties of Spider Silks Revealed by the Complete Gene Sequence of the Prey-Wrapping Silk Protein (AcSp1) [J].
Ayoub, Nadia A. ;
Garb, Jessica E. ;
Kuelbs, Amanda ;
Hayashi, Cheryl Y. .
MOLECULAR BIOLOGY AND EVOLUTION, 2013, 30 (03) :589-601
[10]   The Nephila clavipes genome highlights the diversity of spider silk genes and their complex expression [J].
Babb, Paul L. ;
Lahens, Nicholas F. ;
Correa-Garhwal, Sandra M. ;
Nicholson, David N. ;
Kim, Eun Ji ;
Hogenesch, John B. ;
Kuntner, Matjaz ;
Higgins, Linden ;
Hayashi, Cheryl Y. ;
Agnarsson, Ingi ;
Voight, Benjamin F. .
NATURE GENETICS, 2017, 49 (06) :895-+
12345