Inhibition of bacterial adhesion to live human cells: Activity and cytotoxicity of synthetic mannosides

被引：37

作者：

Hartmann, Mirja ^{[1
]}

Papavlassopoulos, Heike ^{[2
]}

Chandrasekaran, Vijayanand ^{[1
]}

Grabosch, Carsten ^{[1
]}

Beiroth, Femke ^{[1
]}

Lindhorst, Thisbe K. ^{[1
]}

Roehl, Claudia ^{[2
]}

机构：

[1] Univ Kiel, Otto Diels Inst Organ Chem, D-24098 Kiel, Germany

[2] Univ Kiel, Inst Toxicol & Pharmacol Nat Scientists, D-24105 Kiel, Germany

来源：

FEBS LETTERS | 2012年 / 586卷 / 10期

关键词：

Bacterial adhesion; alpha-Mannoside inhibitor; HT-29; cell; Cytotoxicity; Anti-adhesion therapy; Type 1 fimbriated E. coli; UROPATHOGENIC ESCHERICHIA-COLI; URINARY-TRACT-INFECTIONS; FIMH ANTAGONISTS; TYPE-1; FIMBRIAE; LECTIN; RECOGNITION; DESIGN; ASSAY; GLYCOBIOLOGY; LIGANDS;

D O I：

10.1016/j.febslet.2012.03.059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacterial adhesion to glycosylated surfaces is a key issue in human health and disease. Inhibition of bacterial adhesion by suitable carbohydrates could lead to an anti-adhesion therapy as a novel approach against bacterial infections. A selection of five alpha-mannosides has been evaluated as inhibitors of bacterial adhesion to the polysaccharide mannan, as well as to the surface of live human HT-29 cells. Cell toxicity studies were performed to identify the therapeutic window for a potential in vivo-application of the tested carbohydrates. A previously published mannosidic squaric acid diamide was shown to be exceptionally effective as inhibitor of the bacterial lectin FimH. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1459 / 1465

页数：7

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