High fidelity fibre-based physiological sensing deep in tissue

被引：11

作者：

Choudhary, Tushar R. ^{[1
,2
,6
,7
]}

Tanner, Michael G. ^{[2
,3
]}

Megia-Fernandez, Alicia ^{[4
]}

Harrington, Kerrianne ^{[5
]}

Wood, Harry A. ^{[5
]}

Marshall, Adam ^{[2
]}

Zhu, Patricia ^{[4
]}

Chankeshwara, Sunay, V ^{[4
]}

Choudhury, Debaditya ^{[3
]}

Monro, Graham ^{[2
]}

Ucuncu, Muhammed ^{[4
]}

Yu, Fei ^{[5
]}

Duncan, Rory R. ^{[1
]}

Thomson, Robert R. ^{[2
,3
]}

Dhaliwal, Kevin ^{[2
]}

Bradley, Mark ^{[2
,4
]}

机构：

[1] Heriot Watt Univ, Sch Engn & Phys Sci, Inst Biol Chem Biophys & Bioengn, Edinburgh, Midlothian, Scotland

[2] Univ Edinburgh, Ctr Inflammat Res, Queens Med Res Inst, EPSRC Proteus IRC Hub, Edinburgh, Midlothian, Scotland

[3] Heriot Watt Univ, Sch Engn & Phys Sci, Inst Photon & Quantum Sci, SUPA, Edinburgh, Midlothian, Scotland

[4] Univ Edinburgh, Sch Chem, EaStChem, Edinburgh, Midlothian, Scotland

[5] Univ Bath, Ctr Photon & Photon Mat, Dept Phys, Bath, Avon, England

[6] Univ Edinburgh, Roslin Inst, Edinburgh, Midlothian, Scotland

[7] Univ Edinburgh, Royal Dick Sch OfVet Studies, Edinburgh, Midlothian, Scotland

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

基金：

英国工程与自然科学研究理事会;

关键词：

OPTIC PH SENSOR; OXYGEN;

D O I：

10.1038/s41598-019-44077-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Physiological sensing deep in tissue remains a clinical challenge. Here a flexible miniaturised sensing optrode providing a platform to perform minimally invasive in vivo in situ measurements is reported. Silica microspheres covalently coupled with a high density of ratiometrically configured fluorophores were deposited into etched pits on the distal end of a 150 mu m diameter multicore optical fibre. With this platform, photonic measurements of pH and oxygen concentration with high precision in the distal alveolar space of the lung are reported. We demonstrated the phenomenon that high-density deposition of carboxyfluorescein covalently coupled to silica microspheres shows an inverse shift in fluorescence in response to varying pH. This platform delivered fast and accurate measurements (+/- 0.02 pH units and +/- 0.6 mg/L of oxygen), near instantaneous response time and a flexible architecture for addition of multiple sensors.

引用

页数：10

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