Modulation of fluorescence sensing properties of coumarin-based fluorescent probe for H2S and its application in cell imaging

被引:24
作者
Jin, Lei [1 ]
Tan, Xiaoxue [1 ]
Dai, Lihui [1 ]
Bai, Hui [2 ]
Wang, Qingming [1 ]
机构
[1] Yancheng Teachers Univ, Sch Pharm, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 224051, Jiangsu, Peoples R China
[2] Taiyuan Univ Technol, Key Lab Coal Sci & Technol, Minist Educ & Shanxi Prov, Taiyuan 030024, Shanxi, Peoples R China
关键词
H2S; Coumarin-based; Fluorescent probe; Mechanism; Cell imaging; ENDOGENOUS HYDROGEN-SULFIDE; HIGHLY SELECTIVE DETECTION; LIVING CELLS; STRATEGY; SENSORS; BODIPY; ACID;
D O I
10.1016/j.saa.2019.117187
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
A coumarin-based turn on probe, named 3-((E)-3-(1H-indol-3-yl)acryloyl)-2H-chromen-2-one(IAC) was designed and synthesized for the detection of H2S in aqueous medium. IAC showed no obvious red fluorescence in Tris-HCl(pH - 7.2, 60% DMF), a 28.2-fold fluorescence enhancement was found when 40 equiv. H2S was added. Other analytes such as anions, metal ions and GSH, Cys, Gly did no significant fluorescence enhancement at 580 nm to IAC. The red fluorescence enhancement mechanism between IAC and H2S was considered by DFT and ESI-MS. Overall, IAC was successfully applied in cell imaging. (C) 2019 Elsevier B.V. All rights reserved.
引用
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页数:7
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