Isolation and characterization of a gene encoding alpha-tubulin from Candida albicans

被引：4
|
作者：
Daly, S
Yacoub, A
Dundon, W
Mastromei, G
Islam, K
Lorenzetti, R
机构：
[1] GRP LEPETIT SPA, LEPETIT RES CTR, I-21040 GERENZANO, VA, ITALY
[2] UNIV FLORENCE, DEPT ANIM BIOL & GENET, FLORENCE, ITALY
来源：
GENE | 1997年 / 187卷 / 02期
关键词：
complementation; recombinant DNA; nucleotide sequence; amino acid sequence;
D O I：
10.1016/S0378-1119(96)00737-8
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A gene encoding the alpha-tubulin of Candida albicans has been cloned and characterized. Nucleotide sequence analysis reveals the presence of an intron within the structural gene and predicts the synthesis of a polypeptide of 448 amino acid residues. Comparison of nucleotide and amino acid sequences with the Saccharomyces cerevisiae alpha-tubulin encoding genes shows a 75% homology and about 92% similarity respectively. In contrast to S. cerevisiae, C. albicans appears to possess only one gene for alpha-tubulin which is able to functionally complement a S. cerevisiae cold-sensitive tub1 mutant.
引用
收藏
页码：151 / 158
页数：8
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