Isolation and characterization of a gene encoding alpha-tubulin from Candida albicans

被引：4
|
作者：
Daly, S
Yacoub, A
Dundon, W
Mastromei, G
Islam, K
Lorenzetti, R
机构：
[1] GRP LEPETIT SPA, LEPETIT RES CTR, I-21040 GERENZANO, VA, ITALY
[2] UNIV FLORENCE, DEPT ANIM BIOL & GENET, FLORENCE, ITALY
来源：
GENE | 1997年 / 187卷 / 02期
关键词：
complementation; recombinant DNA; nucleotide sequence; amino acid sequence;
D O I：
10.1016/S0378-1119(96)00737-8
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A gene encoding the alpha-tubulin of Candida albicans has been cloned and characterized. Nucleotide sequence analysis reveals the presence of an intron within the structural gene and predicts the synthesis of a polypeptide of 448 amino acid residues. Comparison of nucleotide and amino acid sequences with the Saccharomyces cerevisiae alpha-tubulin encoding genes shows a 75% homology and about 92% similarity respectively. In contrast to S. cerevisiae, C. albicans appears to possess only one gene for alpha-tubulin which is able to functionally complement a S. cerevisiae cold-sensitive tub1 mutant.
引用
收藏
页码：151 / 158
页数：8
相关论文
共 50 条
  • [1] ISOLATION AND CHARACTERIZATION OF AN ALPHA-TUBULIN GENE FROM LEISHMANIA-ENRIETTII
    WIRTH, DF
    SLATER, C
    MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1983, 9 (01) : 83 - 92
  • [2] ISOLATION AND CHARACTERIZATION OF A BETA-TUBULIN GENE FROM CANDIDA-ALBICANS
    SMITH, HA
    ALLAUDEEN, HS
    WHITMAN, MH
    KOLTIN, Y
    GORMAN, JA
    GENE, 1988, 63 (01) : 53 - 63
  • [3] ISOLATION AND CHARACTERIZATION OF 2 RAT ALPHA-TUBULIN ISOTYPES
    GINZBURG, I
    TEICHMAN, A
    LITTAUER, UZ
    ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1986, 466 : 31 - 40
  • [4] ISOLATION AND PRELIMINARY CHARACTERIZATION OF HUMAN ALPHA-TUBULIN AND BETA-TUBULIN GENES
    VENEGAS, A
    VILLANUEVA, J
    BULLI, P
    BELL, G
    VALENZUELA, P
    ARCHIVOS DE BIOLOGIA Y MEDICINA EXPERIMENTALES, 1980, 13 (01): : 116 - 116
  • [5] CLONING AND CHARACTERIZATION OF THE ALTA ALPHA-TUBULIN GENE OF PHYSARUM
    CUNNINGHAM, DB
    BUCHSCHACHER, GL
    BURLAND, TG
    DOVE, WF
    KESSLER, D
    PAUL, ECA
    JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 : 137 - 151
  • [6] Characterization of the gene family encoding alternative oxidase from Candida albicans
    Huh, WK
    Kang, SO
    BIOCHEMICAL JOURNAL, 2001, 356 : 595 - 604
  • [7] CHARACTERIZATION OF THE ALPHA-TUBULIN GENE FAMILY OF ARABIDOPSIS-THALIANA
    LUDWIG, SR
    OPPENHEIMER, DG
    SILFLOW, CD
    SNUSTAD, DP
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (16) : 5833 - 5837
  • [8] THE ISOLATION AND CHARACTERIZATION OF 2 GENES-CODING FOR ALPHA-TUBULIN IN DROSOPHILA
    KALFAYAN, L
    WENSINK, P
    EUROPEAN JOURNAL OF CELL BIOLOGY, 1980, 22 (01) : 14 - 14
  • [9] ISOLATION OF ALPHA-TUBULIN GENES FROM THE HUMAN MALARIA PARASITE, PLASMODIUM-FALCIPARUM - SEQUENCE-ANALYSIS OF ALPHA-TUBULIN
    HOLLOWAY, SP
    SIMS, PFG
    DELVES, CJ
    SCAIFE, JG
    HYDE, JE
    MOLECULAR MICROBIOLOGY, 1989, 3 (11) : 1501 - 1510
  • [10] A CDNA-ENCODING AN ALPHA-TUBULIN FROM SCHISTOSOMA-MANSONI
    WEBSTER, PJ
    SETA, KA
    CHUNG, SC
    MANSOUR, TE
    MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 51 (01) : 169 - 170
12345