Diet Folate, DNA Methylation and Polymorphisms in Methylenetetrahydrofolate Reductase in Association with the Susceptibility to Gastric Cancer

被引：21
作者：
Gao, Shang [1 ]
Ding, Li-Hong [2 ]
Wang, Jian-Wei [1 ]
Li, Cun-Bao [3 ]
Wang, Zhao-Yang [4 ]
机构：
[1] Inner Mongolia Med Univ, Affiliated Hosp, Sch Basic Med, Dept Anthropot, Hohhot, Peoples R China
[2] Inner Mongolia Med Univ, Affiliated Hosp, Dept Internal Med, Hohhot, Peoples R China
[3] Inner Mongolia Med Univ, Affiliated Hosp, Dept Biochem & Mol Biol, Hohhot, Peoples R China
[4] Inner Mongolia Med Univ, Affiliated Hosp, Dept Gen Surg, Hohhot, Peoples R China
来源：
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION | 2013年 / 14卷 / 01期
关键词：
Methylenetetrahydrofolate reductase; DNA methylation; folate intake; gastric cancer risk; SQUAMOUS-CELL CARCINOMA; HELICOBACTER-PYLORI INFECTION; C677T GENETIC-POLYMORPHISM; COLORECTAL-CANCER; HMLH1; GENES; RISK; MGMT; COMBINATION; POPULATION; BREAST;
D O I：
10.7314/APJCP.2013.14.1.299
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Methylenetetrahydrofolate reductase (MTHFR) has been reported to be associated with DNA methylation, an epigenetic feature frequently found in gastric cancer. We conducted a case-control study to explore the association of MTHFR C677T polymorphisms with gastric cancer risk and its relation with the DNA methylation of COX-2, MGMT, and hMLH1 genes. Genotyping of P16, MGMT and HMLH1 was determined by methylation-specific PCR after sodium bisulfate modification of DNA, and genotyping of MTHFR C677T was conducted by TaqMan assays using the ABI Prism 7911HT Sequence Detection System. Folate intake was calculated with the aid of a questionnaire. Compared with the MTHFR 677CC genotype, the TT genotype was significantly associated with 2.08 fold risk of gastric cancer when adjusting for potential risk factors. Individuals who had an intake of folate above 310 mu g/day showed protective effects against gastric cancer risk. The effect of MTHFR C677T polymorphisms on the risk of gastric cancer was modified by folate intake and methylation status of MGMT (P for interaction <0.05).
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页码：299 / 302
页数：4
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