Leucine-rich repeat-containing G-protein-coupled receptor 2 (LGR2) can regulate PO activity and AMP genes expression in Macrobrachium nipponense

被引：6

作者：

Cao, Xueying ^{[1
]}

Zhang, Chao ^{[1
]}

Zhang, Ruidong ^{[1
]}

Wang, Kaiqiang ^{[1
]}

Dai, Xiaoling ^{[1
]}

Huang, Xin ^{[1
]}

Ren, Qian ^{[1
,2
]}

机构：

[1] Nanjing Normal Univ, Jiangsu Prov Engn Res Ctr Aquat Anim Breeding & G, Coll Marine Sci & Engn, 1 Wenyuan Rd, Nanjing 210023, Jiangsu, Peoples R China

[2] Coinnovat Ctr Marine Bioind Technol Jiangsu Prov, Lianyungang 222005, Jiangsu, Peoples R China

来源：

MOLECULAR IMMUNOLOGY | 2020年 / 126卷

基金：

中国国家自然科学基金;

关键词：

LGR2; Anti-microbial peptide (AMP); Relish; Phenol oxidase; Macrobrachium nipponense; CUTICLE-HARDENING HORMONE; NEUROHORMONE GPCRS; BURSICON;

D O I：

10.1016/j.molimm.2020.07.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leucine-rich repeat-containing G-protein-coupled receptors (LGRs) form a subfamily of the large superfamily of G-protein-coupled receptors. LGRs can be divided into three groups. LGR2 from Drosophila melanogaster is involved in cuticle tanning (melanization and sclerotization). In this study, one LGR2 (MnLGR2) was identified from Macrobrachium nipponense. MnLGR2 has an open reading frame of 4515 bp encoding a protein with 1504 amino acids. MnLGR2 is comprised of a 7-transmembrane domain, 12 leucine-rich repeats, and 5 low-complexity regions. The highest expression level of MnLGR2 was observed in gills. The expression levels of MnLGR2 in gills and stomach could be regulated by bacterial challenge. Knockdown of MnLGR2 upregulated the expression of anti-microbial peptide (AMP) genes. Further study indicated that inhibition of AMP expression by MnLGR2 was through inhibition of relish-mediated AMP expression. In addition to the negative regulation of AMP expression, MnLGR2 participated in positive regulation of phenol oxidase (PO) activity and expression of proPO activating pathway-related genes (proPO-activating factor and proPO-activating enzymes). Therefore, MnLGR2 plays an important role in prawn innate immunity.

引用

页码：14 / 24

页数：11

共 22 条

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