Structure and activity of Candida antarctica lipase B in ionic liquids

被引：129

作者：

van Rantwijk, F

Secundo, F

Sheldon, RA

机构：

[1] CNR, Ist Chim Riconoscimento Mol, I-20133 Milan, Italy

[2] Delft Univ Technol, Lab Biocatalysis & Organ Chem, NL-2628 BL Delft, Netherlands

来源：

GREEN CHEMISTRY | 2006年 / 8卷 / 03期

关键词：

D O I：

10.1039/b513062j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The effects of ionic liquid media on the activity of Candida antarctica lipase B in a simple transesterification reaction were studied. In ionic liquids containing dicyanimide, alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF4]. Only [Et3MeN][MeSO4] was an exception, as dissolved CaLB maintained its activity in this solvent. A cross-linked enzyme aggregate of CaLB was twice as active in [BMIm][dca] as in tert-butyl alcohol, whereas the free enzyme was irreversibly deactivated in this latter ionic liquid.

引用

页码：282 / 286

页数：5

共 42 条

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