Porcine mesothelium matrix as a biomaterial for wound healing applications

被引:26
作者
Capella-Monsonis, H. [1 ,2 ]
Tilbury, M. A. [2 ,3 ]
Wall, J. G. [2 ,3 ]
Zeugolis, D., I [1 ,2 ]
机构
[1] Natl Univ Ireland Galway NUI Galway, Regenerat Modular & Dev Engn Lab REMODEL, Galway, Ireland
[2] Natl Univ Ireland Galway NUI Galway, Sci Fdn Ireland SFI, Ctr Res Med Devices CURAM, Galway, Ireland
[3] Natl Univ Ireland Galway NUI Galway, Dept Microbiol, Galway, Ireland
基金
爱尔兰科学基金会;
关键词
Xenografts; Collagen devices; Functional biomaterials; Immune response; Angiogenesis; PROTEASE-MODULATING MATRIX; URINARY-BLADDER MATRIX; DIABETIC FOOT ULCERS; LONG-TERM OUTCOMES; COLLAGEN TYPE-I; EXTRACELLULAR-MATRIX; GROWTH-FACTORS; ACELLULAR MATRIX; FIBRONECTIN; TISSUE;
D O I
10.1016/j.mtbio.2020.100057
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The increasing economic burden of wound healing in healthcare systems requires the development of functional therapies. Xenografts with preserved extracellular matrix (ECM) structure and biofunctional components overcome major limitations of autografts and allografts (e.g. availability) and artificial biomaterials (e.g. foreign body response). Although porcine mesothelium is extensively used in clinical practice, it is under-investigated for wound healing applications. Herein, we compared the biochemical and biological properties of the only two commercially available porcine mesothelium grafts (Meso Biomatrix (R) and Puracol (R) Ultra ECM) to traditionally used wound healing grafts (EndoformTM, ovine forestomach and MatriStem (R), porcine urinary bladder) and biomaterials (PromogranTM, collagen/oxidized regenerated cellulose). The EndoformTM and the Puracol (R) Ultra ECM showed the highest (p<0.05) soluble collagen and elastin content. The MatriStem (R) had the highest (p<0.05) basic fibroblast growth factor (FGFb) content, whereas the Meso Biomatrix (R) had the highest (p 0.05) transforming growth factor beta-1 (TGF-beta 1) and vascular endothelial growth factor (VEGF) content. All materials showed tissue-specific structure and composition. The EndoformTM and the Meso Biomatrix (R) had some nuclei residual matter. All tissue grafts showed similar (p 0.05) response to enzymatic degradation, whereas the PromogranTM was not completely degraded by matrix metalloproteinase (MMP)-8 and was completely degraded by elastase. The PromogranTM showed the highest (p<0.05) permeability to bacterial infiltration. The PromogranTM showed by far the lowest dermal fibroblast and THP-1 attachment and growth. All tested materials showed significantly lower (p<0.05) tumor necrosis factor-alpha (TNF-alpha) expression than the lipopolysaccharides group. The MatriStem (R) and the Puracol (R) Ultra ECM promoted the highest (p<0.05) number of micro-vessel formation, whereas the PromogranTM the lowest (p<0.05). Collectively, these data confer that porcine mesothelium has the potential to be used as a wound healing material, considering its composition, resistance to enzymatic degradation, cytocompatibility, and angiogenic potential.
引用
收藏
页数:12
相关论文
共 50 条
[31]   Surfaces to enhance matrix deposition for wound healing [J].
Fernandez, T. ;
Strudwick, X. L. ;
Al-Bataineh, S. ;
Short, R. D. ;
Cowin, A. J. ;
Whittle, J. D. ;
Smith, L. E. .
WOUND PRACTICE AND RESEARCH, 2018, 26 (04) :201-209
[32]   Electrospinning of natural polymers for the production of nanofibres for wound healing applications [J].
Bombin, Adrian D. Juncos ;
Dunne, Nicholas J. ;
McCarthy, Helen O. .
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2020, 114
[33]   Stabilization of growth factors relevant to wound healing by a plant cell wall biomaterial [J].
Ni, Yawei ;
Turner, Debra ;
Yates, Kenneth ;
Tizard, Ian .
PLANTA MEDICA, 2007, 73 (12) :1260-1266
[34]   A photocrosslinking antibacterial decellularized matrix hydrogel with nanofiber for cutaneous wound healing [J].
Yu, Fan ;
Khan, Atta ur Rehman ;
Zheng, Hui ;
Li, Xiaotong ;
EL-Newehy, Mohamed ;
EL-Hamshary, Hany ;
Morsi, Yosry ;
Li, Jun ;
Wu, Jinglei ;
Mo, Xiumei .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2022, 217
[35]   Fabrication and In Vitro Characterization of Electrochemically Compacted Collagen/Sulfated Xylorhamnoglycuronan Matrix for Wound Healing Applications [J].
Kang, Lingzhi ;
Liu, Xiao ;
Yue, Zhilian ;
Chen, Zhi ;
Baker, Chris ;
Winberg, Pia C. ;
Wallace, Gordon G. .
POLYMERS, 2018, 10 (04)
[36]   Accelerated Porcine Wound Healing after Treatment with α-Gal Nanoparticles [J].
Hurwitz, Zachary M. ;
Ignotz, Ronald ;
Lalikos, Janice F. ;
Galili, Uri .
PLASTIC AND RECONSTRUCTIVE SURGERY, 2012, 129 (02) :242E-251E
[37]   The biological processes during wound healing [J].
Yang, Fan ;
Bai, Xiangjun ;
Dai, Xiaojing ;
Li, Yong .
REGENERATIVE MEDICINE, 2021, 16 (04) :373-390
[38]   PHARMACOLOGICAL ENHANCEMENT OF WOUND-HEALING [J].
PIERCE, GF ;
MUSTOE, TA .
ANNUAL REVIEW OF MEDICINE, 1995, 46 :467-481
[39]   Oxygen Delivery Biomaterials in Wound Healing Applications [J].
Bayraktar, Sema ;
Ustun, Cansu ;
Kehr, Nermin Seda .
MACROMOLECULAR BIOSCIENCE, 2024, 24 (03)
[40]   Hyaluronan in skin wound healing: therapeutic applications [J].
King, Ian C. C. ;
Sorooshian, Parviz .
JOURNAL OF WOUND CARE, 2020, 29 (12) :782-787