Reconstitution in vitro of the entire cycle of the mouse female germ line

被引：417

作者：

Hikabe, Orie ^{[1
]}

Hamazaki, Nobuhiko ^{[1
]}

Nagamatsu, Go ^{[1
]}

Obata, Yayoi ^{[2
]}

Hirao, Yuji ^{[3
]}

Hamada, Norio ^{[1
,4
]}

Shimamoto, So ^{[1
]}

Imamura, Takuya ^{[1
]}

Nakashima, Kinichi ^{[1
]}

Saitou, Mitinori ^{[5
,6
,7
,8
]}

Hayashi, Katsuhiko ^{[1
,9
]}

机构：

[1] Kyushu Univ, Grad Sch Med Sci, Dept Stem Cell Biol & Med, Higashi Ku, Maidashi 3-1-1, Fukuoka 8128582, Japan

[2] Tokyo Univ Agr, Dept Biosci, Setagaya Ku, 1-1-1 Sakuragaoka, Tokyo 1568502, Japan

[3] NARO Inst Livestock & Grassland Sci, Ikenodai 2, Tsukuba, Ibaraki 3050901, Japan

[4] Kyushu Univ, Grad Sch Med Sci, Dept Obstet & Gynecol, Higashi Ku, Maidashi 3-1-1, Fukuoka 8128582, Japan

[5] Kyoto Univ, Grad Sch Med, Dept Anat & Cell Biol, Sakyo Ku, Yoshida Konoe Cho, Kyoto 6068501, Japan

[6] Kyoto Univ, Ctr iPS Cell Res & Applicat, Sakyo Ku, 53 Kawahara Cho, Kyoto 6068507, Japan

[7] Kyoto Univ, Inst Integrated Cell Mat Sci, Sakyo Ku, Yoshida Ushinomiya Cho, Kyoto 6068501, Japan

[8] JST, ERATO, Sakyo Ku, Yoshida Konoe Cho, Kyoto 6068501, Japan

[9] JST, PRESTO, Higashi Ku, Maidashi 3-1-1, Fukuoka 8128582, Japan

来源：

NATURE | 2016年 / 539卷 / 7628期

关键词：

EMBRYONIC STEM-CELLS; OOCYTES; GENE; MICE; QUANTIFICATION; GENERATION; INDUCTION; MEMORY; OVARY; FATE;

D O I：

10.1038/nature20104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The female germ line undergoes a unique sequence of differentiation processes that confers totipotency to the egg(1,2). The reconstitution of these events in vitro using pluripotent stem cells is a key achievement in reproductive biology and regenerative medicine. Here we report successful reconstitution in vitro of the entire process of oogenesis from mouse pluripotent stem cells. Fully potent mature oocytes were generated in culture from embryonic stem cells and from induced pluripotent stem cells derived from both embryonic fibroblasts and adult tail tip fibroblasts. Moreover, pluripotent stem cell lines were re-derived from the eggs that were generated in vitro, thereby reconstituting the full female germline cycle in a dish. This culture system will provide a platform for elucidating the molecular mechanisms underlying totipotency and the production of oocytes of other mammalian species in culture.

引用

页码：299 / +

页数：17

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