Determination of ochratoxin A in wines by capillary liquid chromatography with laser induced fluorescence detection using dispersive liquid-liquid microextraction

被引：67
作者：
Arroyo-Manzanares, Natalia [1 ]
Gamiz-Gracia, Laura [1 ]
Garcia-Campana, Ana M. [1 ]
机构：
[1] Univ Granada, Dept Analyt Chem, Fac Sci, E-18071 Granada, Spain
来源：
FOOD CHEMISTRY | 2012年 / 135卷 / 02期
关键词：
Ochratoxin A; Capillary-HPLC; Laser induced fluorescence detection; Dispersive liquid-liquid microextraction; Wine; MASS-SPECTROMETRY; SAMPLES; ELECTROPHORESIS; QUANTIFICATION; EXTRACTION; ASSAY; BEER; COOCCURRENCE; MYCOTOXINS; AFLATOXINS;
D O I：
10.1016/j.foodchem.2012.05.009
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A method based on reverse phase capillary high performance liquid chromatography (capillary HPLC) coupled to laser-induced fluorescence detection (LIF) has been proposed for the determination of ochratoxin A (OTA) in wine samples. An anionic micellar medium was added to the mobile phase for increasing the fluorescence intensity and peak efficiency. Dispersive liquid-liquid microextraction (DLLME) has been used as a simple and efficient sample pretreatment method for the analysis of OTA in wines, being optimised by means of experimental design. The limit of detection was 5.5 ng L-1 (3 x S/N) and recoveries for different wines ranged from 91.7 to 98.1%. The proposed methodology could be classified as a green analytical chemistry alternative, combining the low organic solvent volumes required in the DLLME with the reduced consumption of mobile phase in capillary HPLC. The use of LIF as detector provided an extremely sensitive method for the determination of OTA in wines. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：368 / 372
页数：5
相关论文
共 32 条
[1]   Combined use of supported liquid membrane and solid-phase extraction to enhance selectivity and sensitivity in capillary electrophoresis for the determination of ochratoxin A in wine [J].
Almeda, Sara ;
Arce, Lourdes ;
Valcarcel, Miguel .
ELECTROPHORESIS, 2008, 29 (07) :1573-1581
[2]   Incidence of ochratoxin A in dried fruits and co-occurrence with aflatoxins in dried figs [J].
Bircan, Cavit .
FOOD AND CHEMICAL TOXICOLOGY, 2009, 47 (08) :1996-2001
[3]   Dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-tandem mass spectrometry for the identification and the accurate quantification by isotope dilution assay of Ochratoxin A in wine samples [J].
Campone, Luca ;
Piccinelli, Anna Lisa ;
Rastrelli, Luca .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 399 (03) :1279-1286
[4]   An Overview of Conventional and Emerging Analytical Methods for the Determination of Mycotoxins [J].
Cigic, Irena Kralj ;
Prosen, Helena .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2009, 10 (01) :62-115
[5]   Capillary electrophoresis with laser-induced fluorescence: Method for the mycotoxin ochratoxin A [J].
Corneli, S ;
Maragos, CM .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (08) :3162-3165
[6]   A review on ochratoxin A occurrence and effects of processing of cereal and cereal derived food products [J].
Duarte, S. C. ;
Pena, A. ;
Lino, C. M. .
FOOD MICROBIOLOGY, 2010, 27 (02) :187-198
[7]  
European Committee for Standardization (CEN), 2003, 141332003 CEN EN EUR
[8]   Simple liquid chromatography assay for analyzing ochratoxin A in bovine milk [J].
González-Osnaya, L. ;
Soriano, J. M. ;
Moltó, J. C. ;
Mañes, J. .
FOOD CHEMISTRY, 2008, 108 (01) :272-276
[9]   Comparison between capillary electrophoresis and HPLC-FL for ochratoxin A quantification in wine [J].
González-Peñas, E ;
Leache, C ;
de Cerain, AL ;
Lizarraga, E .
FOOD CHEMISTRY, 2006, 97 (02) :349-354
[10]   Validation of two analytical methods for the determination of ochratoxin A by reversed-phased high-performance liquid chromatography coupled to fluorescence detection in musts and sweet wines from Andalusia [J].
Hernández, MJ ;
García-Moreno, MV ;
Durán, E ;
Guillén, D ;
Barroso, CG .
ANALYTICA CHIMICA ACTA, 2006, 566 (01) :117-121
1234