The rapid identification of isoflavonoids from Belamcanda chinensis by LC-NMR and LG-MS

被引：29

作者：

Kang, Suk Woo ^{[1
]}

Kim, Min Cheol ^{[1
]}

Kim, Chul Young ^{[1
]}

Jung, Sang Hoon ^{[1
]}

Um, Byung Hun ^{[1
]}

机构：

[1] Korea Inst Sci & Technol, Nat Prod Res Ctr, Gangneung Inst, Kangnung 210340, South Korea

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2008年 / 56卷 / 10期

关键词：

LC hyphenated technique; LC-NMR; LC-MS; isoflavonoid; Belameanda chinensis;

D O I：

10.1248/cpb.56.1452

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The use of hyphenated LC-NMR and LC-MS techniques for the purpose of directly identifying the major constituents of Belamcanda chinensis was investigated. Reversed-phase isocratic chromatography performed using an acetonitrile-water solvent system on a C18 column. The NMR spectrum yielded five main peaks, whose analysis revealed them to he 5, 6, 7, 3'-tetrahydroxy-4'-methoxyisoflavone (1), tectorioenin (2), iristectorigenin A (3), irigenin (4), and irisflorentine (5). The identification of these constituents was confirmed by performing LC-ESI-MS experiment. This study shows that hyphenated LC-NMR and LC-MS can he used for the rapid (70 min) identification of the isoflavonoids.

引用

页码：1452 / 1454

页数：3

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