Cloning and sequencing of the Pz-peptidase gene from Bacillus licheniformis N22

被引：7

作者：

Akiyama, K ^{[1
]}

Mori, K ^{[1
]}

Takata, R ^{[1
]}

机构：

[1] Ehime Univ, Fac Agr, Matsuyama, Ehime 7908566, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 1999年 / 87卷 / 02期

关键词：

Pz-peptidase; Bacillus licheniformis; cloning;

D O I：

10.1016/S1389-1723(99)89018-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pz-peptidase is an endopeptidase that cleaves the synthetic substrate Pt-peptide (4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg), which was originally developed for the assay of collagenase. The Pt-peptidase gene of Bacillus licheniformis N22 was cloned and sequenced. The gene consists of 628 amino acids with a motif for zinc-dependent metalloprotease, and shares 42% amino acid identity with the oligoendopeptidase of Lactococcus lactis. This is the first report on the gene structure of a Pz-peptidase.

引用

页码：231 / 233

页数：3

共 18 条

[1] ISOLATION AND CHARACTERIZATION OF PZ-PEPTIDASE FROM BACILLUS-LICHENIFORMIS N22 [J].