Changes in the protein composition of cytoplasmic ribosomes during the greening of etiolated barley leaves

被引：0

作者：

Koyama, K ^{[1
]}

Wada, A ^{[1
]}

Maki, Y ^{[1
]}

Tanaka, A ^{[1
]}

机构：

[1] KYOTO UNIV, FAC SCI, DEPT PHYS, KYOTO 60601, JAPAN

来源：

PHYSIOLOGIA PLANTARUM | 1996年 / 96卷 / 01期

关键词：

barley; greening; Hordeum vulgare; ribosomal protein; ribosome;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We examined changes in the protein composition of cytoplasmic ribosomes in etiolated barley leaves following illumination. Cytoplasmic ribosomes were isolated from greening barley leaves by sucrose density gradient centrifugation, and were analyzed by radical-free highly reducing polyacrylamide gel electrophoresis (RFHR-PAGE). Eighty-nine proteins were resolved from the ribosomal fraction; among them, 8 proteins changed their copy numbers depending on the stage of greening. We designated these as phase dependent ribosomal proteins (PD1-PD8). Two of the proteins (PD1 and 5) present in the ribosomes of etiolated leaves showed a decrease in level during greening. In contrast, the levels of 6 ribosomal proteins (PD2, 3, 4, 6, 7 and 8) increased as greening proceeded. N-terminal amino acid sequence of PD8 showed high homology to rat ribosomal protein L34. The ribosomal proteins that appeared after illumination were not found in any fraction of the etiolated leaves, suggesting that they were synthesized after the onset of illumination. Copy numbers of other ribosomal proteins did not change during greening.

引用

页码：85 / 90

页数：6

共 25 条

[1] THE PRIMARY STRUCTURE OF RAT RIBOSOMAL PROTEIN-L34 [J].