Use of Bordetella pertussis BP3385 To Establish a Cutoff Value for an IS481-Targeted Real-Time PCR Assay

被引：59

作者：

Guthrie, J. L. ^{[1
]}

Seah, C. ^{[1
]}

Brown, S. ^{[1
]}

Tang, P. ^{[1
]}

Jamieson, F. ^{[1
,2
]}

Drews, S. J. ^{[1
,2
,3
]}

机构：

[1] Minist Hlth & Long Term Care, Ontario Publ Hlth Labs, Toronto, ON M9P 3T1, Canada

[2] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[3] Mt Sinai Hosp, Toronto, ON M5G 1X5, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 11期

关键词：

D O I：

10.1128/JCM.01551-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C-T) values of >35. IS481 PCRs with C-T values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.

引用

页码：3798 / 3799

页数：2

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