Use of Bordetella pertussis BP3385 To Establish a Cutoff Value for an IS481-Targeted Real-Time PCR Assay

被引：59

作者：

Guthrie, J. L. ^{[1
]}

Seah, C. ^{[1
]}

Brown, S. ^{[1
]}

Tang, P. ^{[1
]}

Jamieson, F. ^{[1
,2
]}

Drews, S. J. ^{[1
,2
,3
]}

机构：

[1] Minist Hlth & Long Term Care, Ontario Publ Hlth Labs, Toronto, ON M9P 3T1, Canada

[2] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[3] Mt Sinai Hosp, Toronto, ON M5G 1X5, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 11期

关键词：

D O I：

10.1128/JCM.01551-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C-T) values of >35. IS481 PCRs with C-T values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.

引用

页码：3798 / 3799

页数：2

共 9 条

[1] What is new in pertussis? [J].

Bamberger, Ellen S. ;

Srugo, Isaac .

EUROPEAN JOURNAL OF PEDIATRICS, 2008, 167 (02) :133-139

[2] Bordetella species are distinguished by patterns of substantial gene loss and host adaptation [J].

Cummings, CA ;

Brinig, MM ;

Lepp, PW ;

van de Pas, S ;

Relman, DA .

JOURNAL OF BACTERIOLOGY, 2004, 186 (05) :1484-1492

[3] Comparison of culture and PCR for detection of Bordetella pertussis and Bordetella parapertussis under routine laboratory conditions [J].

Dragsted, DM ;

Dohn, B ;

Madsen, J ;

Jensen, JS .

JOURNAL OF MEDICAL MICROBIOLOGY, 2004, 53 (08) :749-754

[4] Outcomes of Bordetella infections in vaccinated children:: Effects of bacterial number in the nasopharynx and patient age [J].

He, QS ;

Arvilommi, H ;

Viljanen, MK ;

Mertsola, J .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1999, 6 (04) :534-536

[5] An outbreak of pertussis among young Israeli soldiers [J].

Klement, E ;

Uliel, L ;

Engel, I ;

Hasin, T ;

Yavzorvi, M ;

Orr, N ;

Davidovitz, N ;

Lahat, N ;

Srugo, I ;

Zangvil, E ;

Cohen, D .

EPIDEMIOLOGY AND INFECTION, 2003, 131 (03) :1049-1054

[6]

Kösters K, 2001, J MED MICROBIOL, V50, P436, DOI 10.1099/0022-1317-50-5-436

[7] Evaluation of PCR for diagnosis of Bordetella pertussis and Bordetella parapertussis infections [J].

Lind-Brandberg, L ;

Welinder-Olsson, C ;

Lagergård, T ;

Taranger, J ;

Trollfors, B ;

Zackrisson, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (03) :679-683

[8] Comparison of PCR, culture, and direct fluorescent-antibody testing for detection of Bordetella pertussis [J].

Loeffelholz, MJ ;

Thompson, CJ ;

Long, KS ;

Gilchrist, MJR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (09) :2872-2876

[9] Pertussis infection in fully vaccinated children in day-care centers, Israel [J].

Srugo, I ;

Benilevi, D ;

Madeb, R ;

Shapiro, S ;

Shohat, T ;

Somekh, E ;

Rimmar, Y ;

Gershtein, V ;

Gershtein, R ;

Marva, E ;

Lahat, N .

EMERGING INFECTIOUS DISEASES, 2000, 6 (05) :526-529

← 1 →