Runx1 is involved in the fusion of the primary and the secondary palatal shelves

被引：26

作者：

Charoenchaikorn, Kesinee ^{[2
]}

Yokomizo, Tomomasa ^{[3
]}

Rice, David P. ^{[4
,5
,6
,7
]}

Honjo, Tadashi ^{[1
]}

Matsuzaki, Kiyomi ^{[2
]}

Shintaku, Yuko ^{[2
]}

Imai, Yuichi ^{[1
]}

Wakamatsu, Asami ^{[3
]}

Takahashi, Satoru ^{[3
]}

Ito, Yoshiaki ^{[8
,9
]}

Takano-Yamamoto, Teruko ^{[10
]}

Thesleff, Irma ^{[11
]}

Yamamoto, Masayuki ^{[12
,13
,14
]}

Yamashiro, Takashi ^{[1
,2
]}

机构：

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthodont & Dentofacial Orthoped, Okayama 7008525, Japan

[2] Osaka Univ, Grad Sch Dent, Dept Orthodont & Dentofacial Orthoped, Suita, Osaka, Japan

[3] Univ Tsukuba, Grad Sch Comprehens Human Sci, Dept Anat & Embryol Biomol & Integrated Med Sci, Tsukuba, Ibaraki, Japan

[4] Univ Helsinki, Inst Dent, Dept Orthodont, Helsinki, Finland

[5] Univ Helsinki, Helsinki Univ Cent Hosp, Helsinki, Finland

[6] Kings Coll London, Dept Craniofacial Dev, London WC2R 2LS, England

[7] Kings Coll London, Dept Orthodont, London WC2R 2LS, England

[8] Natl Univ Singapore, Inst Mol & Cell Biol, Singapore 117548, Singapore

[9] Oncol Res Inst, Singapore, Singapore

[10] Tohoku Univ, Grad Sch Dent, Div Orthodont & Dentofacial Orthoped, Sendai, Miyagi 980, Japan

[11] Univ Helsinki, Inst Biotechnol, Dev Biol Programme, Helsinki, Finland

[12] Univ Tsukuba, Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki, Japan

[13] Univ Tsukuba, ERATO, JST, Environm Response Project, Tsukuba, Ibaraki, Japan

[14] Tohoku Univ, Grad Sch Med, Dept Med Biochem, Sendai, Miyagi 980, Japan

来源：

DEVELOPMENTAL BIOLOGY | 2009年 / 326卷 / 02期

基金：

日本学术振兴会;

关键词：

Runx1; Fgf18; Cleft palate; Palatogenesis; Anterior clefting; Primary palate; Secondary palate; Epithelial fusion; Epithelial-mesenchymal interactions; Palatal explants; TRANSFORMING GROWTH FACTOR-BETA-3; EDGE EPITHELIAL-CELLS; CLEFT-PALATE; TRANSCRIPTION FACTORS; P63; GENE; CONGENITAL-MALFORMATIONS; EEC-SYNDROME; EXPRESSION; DIFFERENTIATION; MICE;

D O I：

10.1016/j.ydbio.2008.10.018

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Runx1 is expressed in medial edge epithelial (MEE) cells of the palatal shelf. Conditionally rescued Runx1(-/-) mice showed limited clefting in the anterior junction between the primary and the secondary palatal shelves, but not in the junction between the secondary palates. In wild type mice, the fusing epithelial surface exhibited a rounded cobblestone-like appearance, while such cellular prominence was less evident in the Runx1 mutants. We also found that Fgf18 was expressed in the mesenchyme underlying the MEE and that locally applied FGF18 induced ectopic Runx1 expression in the epithelium of the palatal explants, indicating that Runx1 was induced by mesenchymal Fgf18 signaling. On the other hand, unpaired palatal explant cultures revealed the presence of anterior-posterior (A-P) differences in the MEE fates and fusion mechanism. Interestingly, the location of anterior clefting in Runx1 mutants corresponded to the region with different MEE behavior. These data showed a novel function of Runx1 in morphological changes in the MEE cells in palatal fusion, which is, at least in part. regulated by the mesenchymal Fgf signaling via an epithelial mesenchymal interaction. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：392 / 402

页数：11

共 64 条

[1] Runx2 mediates FGF signaling from epithelium to mesenchyme during tooth morphogenesis [J].

Åberg, T ;

Wang, XP ;

Kim, JH ;

Yamashiro, T ;

Bei, M ;

Rice, R ;

Ryoo, HM ;

Thesleff, I .

DEVELOPMENTAL BIOLOGY, 2004, 270 (01) :76-93

[2]

Aberg T, 1997, DEV DYNAM, V210, P383, DOI 10.1002/(SICI)1097-0177(199712)210:4<383::AID-AJA3>3.0.CO

[3]

2-C

[4] The cellular and molecular etiology of the cleft secondary palate in Fgf10 mutant mice [J].

Alappat, SR ;

Zhang, ZY ;

Suzuki, K ;

Zhang, XY ;

Liu, HB ;

Jiang, RL ;

Yamada, G ;

Chen, YP .

DEVELOPMENTAL BIOLOGY, 2005, 277 (01) :102-113

[5] Analysis of the p63 gene in classical EEC syndrome, related syndromes, and non-syndromic orofacial clefts [J].

Barrow, LL ;

van Bokhoven, H ;

Daack-Hirsch, S ;

Andersen, T ;

van Beersum, SEC ;

Gorlin, R ;

Murray, JC .

JOURNAL OF MEDICAL GENETICS, 2002, 39 (08) :559-566

[6]

Britto JA, 2002, CLEFT PALATE-CRAN J, V39, P332, DOI 10.1597/1545-1569(2002)039<0332:TPOACP>2.0.CO

[7]

[8] Heterozygous germline mutations in the p53 homolog p63 are the cause of EEC syndrome [J].

Celli, J ;

Duijf, P ;

Hamel, BCJ ;

Bamshad, M ;

Kramer, B ;

Smits, APT ;

Newbury-Ecob, R ;

Hennekam, RCM ;

Van Buggenhout, G ;

van Haeringen, B ;

Woods, CG ;

van Essen, AJ ;

de Waal, R ;

Vriend, G ;

Haber, DA ;

Yang, A ;

McKeon, F ;

Brunner, HG ;

van Bokhoven, H .

CELL, 1999, 99 (02) :143-153

[9] Runx1 determines nociceptive sensory neuron phenotype and is required for thermal and neuropathic pain [J].

Chen, CL ;

Broom, DC ;

Liu, Y ;

de Nooij, JC ;

Li, Z ;

Cen, CA ;

Samad, OA ;

Jessell, TM ;

Woolf, CJ ;

Ma, QF .

NEURON, 2006, 49 (03) :365-377

[10] Overexpression of Smad2 in Tgf-β3-null mutant mice rescues cleft palate [J].

Cui, XM ;

Shiomi, N ;

Chen, JC ;

Saito, T ;

Yamamoto, T ;

Ito, Y ;

Bringas, P ;

Chat, Y ;

Shuler, CF .

DEVELOPMENTAL BIOLOGY, 2005, 278 (01) :193-202

← 1 2 3 4 5 6 7 →