A new paradigm for transcription factor TFIIB functionality

被引：15

作者：

Gelev, Vladimir ^{[1
]}

Zabolotny, Janice M. ^{[1
]}

Lange, Martin ^{[1
]}

Hiromura, Makoto ^{[1
]}

Yoo, Sang Wook ^{[1
]}

Orlando, Joseph S. ^{[2
]}

Kushnir, Anna ^{[2
]}

Horikoshi, Nobuo ^{[1
]}

Paquet, Eric ^{[3
]}

Bachvarov, Dimcho ^{[3
]}

Schaffer, Priscilla A. ^{[2
]}

Usheva, Anny ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Dept Med, Boston, MA 02215 USA

[2] Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Dept Microbiol & Mol Genet, Boston, MA 02215 USA

[3] Univ Laval, Hop Hotel Dieu Quebec, Ctr Hosp Univ Quebec CHUQ Ctr Rech, Quebec City, PQ G1R 2J6, Canada

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

关键词：

RNA-POLYMERASE-II; INITIATION-FACTOR IIB; TATA-BINDING PROTEIN; SIMPLEX-VIRUS TYPE-1; CORE PROMOTER; BASAL TRANSCRIPTION; GENE-TRANSCRIPTION; DNA; TBP; ASSOCIATION;

D O I：

10.1038/srep03664

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Experimental and bioinformatic studies of transcription initiation by RNA polymerase II (RNAP2) have revealed a mechanism of RNAP2 transcription initiation less uniform across gene promoters than initially thought. However, the general transcription factor TFIIB is presumed to be universally required for RNAP2 transcription initiation. Based on bioinformatic analysis of data and effects of TFIIB knockdown in primary and transformed cell lines on cellular functionality and global gene expression, we report that TFIIB is dispensable for transcription of many human promoters, but is essential for herpes simplex virus-1 (HSV-1) gene transcription and replication. We report a novel cell cycle TFIIB regulation and localization of the acetylated TFIIB variant on the transcriptionally silent mitotic chromatids. Taken together, these results establish a new paradigm for TFIIB functionality in human gene expression, which when downregulated has potent anti-viral effects.

引用

页数：8

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