Human CD34+ cells differentiate into microglia and express recombinant therapeutic protein

被引：129
作者：
Asheuer, M
Pflumio, FO
Benhamida, S
Dubart-Kupperschmitt, A
Fouquet, F
Imai, Y
Aubourg, P
Cartier, N [1 ]
机构：
[1] Hop St Vincent de Paul, INSERM U561, F-75014 Paris, France
[2] Natl Inst Neurosci, Dept Neurochem, Tokyo, Japan
[3] Inst Cochin Genet Mol, INSERM U567, Dept Hematol, F-75014 Paris, France
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2004年 / 101卷 / 10期
关键词：
D O I：
10.1073/pnas.0306431101
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In rodents, bone marrow-derived cells enter the brain during adult life. Allogeneic bone marrow transplantation is used to treat genetic CNS diseases, but the fate of human bone marrow and CD34(+) cells within the brain remains to be elucidated. The present study demonstrates that cells derived from human CD34(+) cells, isolated from either cord blood or peripheral blood, migrate into the brain after infusion into nonobese diabetic/severe combined immunodeficient mice. Both types of CD34(+)-derived cells differentiate into perivascular and ramified microglia. The lentiviral transfer of genes into CD34(+) cells before infusion does not modify the differentiation of human CD34(+) cells into microglia, allowing new transgenic proteins to be expressed in these cells. The transplantation of CD34(+) cells could thus be used for the treatment of CNS diseases.
引用
收藏
页码：3557 / 3562
页数：6
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