In Vitro Shoot Regeneration from Stem Internodes of Polygonum tinctorium

An efficient and simple protocol for shoot organogenesis and plant regeneration from the stem internodes of Polygonum tinctorium was developed. The stem internodes were cultured on media containing different concentrations of cytokinins. Thereafter, the optimum concentration of cytokinin was selected and supplemented with various concentrations of auxins, to determine the potential regeneration capacity and shoot growth from excised stem internodes. Of the cytokinins, 6-benzylaminopurine exhibited the highest efficiency for shoot initiation, followed by zeatin, kinetin, and 1-phenyl-3-(1, 2, 3-thiadiazol-5-yl)-urea. In this study, 2.0 mg/L of 6-benzylaminopurine was highly effective for shoot initiation, in terms of regeneration efficiency, shoot number, and shoot length. Among the cytokinin/ auxin combinations, 2.0 mg/L of 6-benzylaminopurine with 0.5 mg/L indole-3-butyric acid provided the optimal conditions for yielding the highest number of shoots (5.6) per explant, greatest shoot length (19.8 mm), and the highest regeneration rate (98%). The protocol developed here is potentially useful for studying the in vitro organogenesis of P. tinctorium. [Thwe AA, Chae SC, Park SU. In Vitro Shoot Regeneration from Stem Internodes of Polygonum tinctorium. Life Sci J 2012;9( 4):2059-2062] (ISSN:1097-8135). http://www.lifesciencesite.com. 308