Nucleic acid extraction methods from fixed and paraffin-embedded tissues in cancer diagnostics

被引：0

作者：

Bonin, Serena ^{[1
]}

Stanta, Giorgio ^{[1
]}

机构：

[1] Univ Trieste, DSM Dept, Cattinara Hosp, I-34149 Trieste, Italy

来源：

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS | 2013年 / 13卷 / 03期

关键词：

automation; commercial kits; DNA; extraction; formalin-fixed paraffin-embedded tissues; quality control; RNA; tissue processing; AUTOMATED DNA EXTRACTION; RNA EXPRESSION ANALYSIS; PROTEOMIC ANALYSIS; GENE-EXPRESSION; FORMALIN FIXATION; QUALITY; PATHOLOGY; RECOVERY; PCR; OPTIMIZATION;

D O I：

10.1586/ERM.13.14

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Diagnostic tests, based on nucleic acid extracts from formalin-fixed and paraffin-embedded tissues, are now becoming increasingly common due to the introduction of biological agents for cancer therapy. Unfortunately, the formalin-fixed and paraffin-embedded tissues are heterogeneous in terms of processing and tissue type, and this has an impact on downstream molecular techniques, especially RNA-based techniques. The present review deals with most of the variables connected to the extraction of nucleic acids from formalin-fixed paraffin-embedded tissues, ranging from tissue processing to quality control of extracts. The most recent peer-reviewed publications (mostly published in the past 5 years) and information provided by company websites have been analyzed to compile this review.

引用

页码：271 / 282

页数：12

共 97 条

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