Development of two culture media for mass cultivation of Azospirillum spp. and for production of inoculants to enhance plant growth

被引：44

作者：

Bashan, Yoav ^{[1
,2
]}

Trejo, Adan ^{[1
]}

de-Bashan, Luz E. ^{[1
,2
]}

机构：

[1] NW Ctr Biol Res CIBNOR, Environm Microbiol Grp, La Paz 23090, Califfornia Sur, Mexico

[2] Bashan Fdn, Corvallis, OR 97330 USA

来源：

BIOLOGY AND FERTILITY OF SOILS | 2011年 / 47卷 / 08期

关键词：

Azospirillum; Biofertilizer; Culture medium; Inoculant; Mass propagation; Plant growth-promoting bacteria; TARGETED OLIGONUCLEOTIDE PROBES; POLY-BETA-HYDROXYBUTYRATE; PROMOTING BACTERIA; BRASILENSE; ASSOCIATION; SUCCINATE; FIELD;

D O I：

10.1007/s00374-011-0555-3

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

High yield culture medium is fundamental for production of inoculants for plant growth-promoting bacteria. Based on substitution of glucose in tryptone-yeast extract-glucose medium by Na-gluconate or glycerol, two new culture media were developed for mass cultivation of the commonly used plant growth-promoting bacterium Azospirillum sp. After 18 h of incubation, these modifications increased populations of different strains of Azospirillum (to similar to 10(11) cells ml(-1) [single cell count] and similar to 5 x 10(9) CFU ml(-1) [plate count method]), significantly reduced generation time, and were also suitable for production of common synthetic inoculants.

引用

页码：963 / 969

页数：7

共 38 条

[1] COMBINATION OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES WITH FLOW-CYTOMETRY FOR ANALYZING MIXED MICROBIAL-POPULATIONS [J].

AMANN, RI ;

BINDER, BJ ;

OLSON, RJ ;

CHISHOLM, SW ;

DEVEREUX, R ;

STAHL, DA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (06) :1919-1925

[2] EFFECT ON WHEAT ROOT DEVELOPMENT OF INOCULATION WITH AN AZOSPIRILLUM-BRASILENSE MUTANT WITH ALTERED INDOLE-3-ACETIC-ACID PRODUCTION [J].

BARBIERI, P ;

GALLI, E .

RESEARCH IN MICROBIOLOGY, 1993, 144 (01) :69-75

[3]

Bashan Y, 2004, CAN J MICROBIOL, V50, P521, DOI [10.1139/w04-035, 10.1139/W04-035]

[4] Inoculants of plant growth-promoting bacteria for use in agriculture [J].

Bashan, Y .

BIOTECHNOLOGY ADVANCES, 1998, 16 (04) :729-770

[5] Alginate microbeads as inoculant carriers for plant growth-promoting bacteria [J].

Bashan, Y ;

Hernandez, JP ;

Leyva, LA ;

Bacilio, M .

BIOLOGY AND FERTILITY OF SOILS, 2002, 35 (05) :359-368

[6] Long-term survival of the plant-growth-promoting bacteria Azospirillum brasilense and Pseudomonas fluorescens in dry alginate inoculant [J].

Bashan, Y ;

Gonzalez, LE .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 51 (02) :262-266

[7] AN IMPROVED SELECTION TECHNIQUE AND MEDIUM FOR THE ISOLATION AND ENUMERATION OF AZOSPIRILLUM-BRASILENSE [J].

BASHAN, Y ;

LEVANONY, H .

CANADIAN JOURNAL OF MICROBIOLOGY, 1985, 31 (10) :947-952

[8]

Bashan Yoav, 1993, P331

[9] HOW THE PLANT GROWTH-PROMOTING BACTERIUM AZOSPIRILLUM PROMOTES PLANT GROWTH-A CRITICAL ASSESSMENT [J].

Bashan, Yoav ;

de-Bashan, Luz E. .

ADVANCES IN AGRONOMY, VOL 108, 2010, 108 :77-136

[10] Enhanced establishment and growth of giant cardon cactus in an eroded field in the Sonoran Desert using native legume trees as nurse plants aided by plant growth-promoting microorganisms and compost [J].

Bashan, Yoav ;

Salazar, Bernardo ;

Esther Puente, Ma. ;

Bacilio, Macario ;

Linderman, Robert .

BIOLOGY AND FERTILITY OF SOILS, 2009, 45 (06) :585-594

← 1 2 3 4 →