Fabrication of chondrocytes/chondrocyte-microtissues laden fibrin gel auricular scaffold for microtia reconstruction

被引:12
作者
Yue, Haiqiong [1 ]
Pathak, Janak L. [1 ]
Zou, Rui [1 ]
Qin, Lei [1 ]
Liao, Ting [1 ]
Hu, Yongxin [1 ]
Kuang, Wei [1 ]
Zhou, Libin [1 ,2 ]
机构
[1] Guangzhou Med Univ, Guangzhou Key Lab Basic & Appl Res Oral Regenerat, Affiliated Stomatol Hosp, Dept Oral & Maxillofacial Surg, Guangzhou, Guangdong, Peoples R China
[2] 306th Hosp PLA, Dept Otolaryngol Head & Neck Surg, Beijing, Peoples R China
关键词
Microtia reconstruction; chondrocytes; microtissues; fibrin gel; auricular mold; cartilaginous tissue; MECHANICAL-PROPERTIES; PERSONAL-EXPERIENCE; EAR RECONSTRUCTION; CELL SHEETS; CARTILAGE; AURICLE; REGENERATION; SEALANTS;
D O I
10.1177/0885328220954415
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Fibrin gel-based scaffolds have promising potential for microtia reconstruction. Autologous chondrocytes and chondrocyte cell sheets are frequently used seed cell sources for cartilage tissue engineering. However, the aesthetic outcome of chondrocyte-based microtia reconstruction is still not satisfactory. In this study, we aimed to fabricate the chondrocytes/chondrocyte-microtissues laden fibrin gel auricular scaffold for microtia reconstruction. We designed a unique auricular mold that could fabricate a fibrin gel scaffold resembling human auricle anatomy. Primary chondrocytes were harvested from rabbit auricular cartilage, and chondrocyte cell sheets were developed. Chondrocyte-microtissues were prepared from the cell sheets. The mixture of chondrocytes/chondrocyte-microtissues was laden in fibrin gel during the auricular scaffold fabrication. The protrusions and recessed structure in the auricular scaffold surface were still clearly distinguishable. After a one-week in vitro culture, the 3 D structure and auricular anatomy of the scaffold were retained. And followed by eight-week subcutaneous implantation, cartilaginous tissue was regenerated in the artificial auricular structure as indicated by the results of H&E, Toluidine blue, Safranin O, and type II collagen (immunohistochemistry) staining. Protrusions and depressions of the auricular scaffold were slightly deformed, but the overall auricular anatomy was maintained after 8-week in vivo implantation. Extracellular matrix components content were similar in artificial auricular cartilage and rabbit native auricular cartilage. In conclusion, the mixture of chondrocytes/chondrocyte-microtissues laden fibrin gel auricular scaffold showed a promising potential for cartilaginous tissue regeneration, suggesting this as an effective approach for autologous chondrocyte-based microtia reconstruction.
引用
收藏
页码:838 / 848
页数:11
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