Chemiluminescence-based electrophoretic mobility shift assay of RNA-protein interactions: Application to binding of viral capsid proteins to RNA

被引：10

作者：

Kang, J

Lee, MS

Watowich, SJ

Gorenstein, DG ^{[1
]}

机构：

[1] Univ Texas, Med Branch, Sealy Ctr Struct Biol, Galveston, TX 77555 USA

[2] Univ Texas, Med Branch, Dept Human Biol Chem & Genet, Galveston, TX 77555 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2006年 / 131卷 / 02期

关键词：

chemiluminescence; EMSA; RNA; Venezuelan equine encephalitis virus; capsid;

D O I：

10.1016/j.jviromet.2005.08.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A chemiluminescence, electrophoretic mobility shift assay was introduced for the study of RNA-protein interactions that include association of genomic RNA and viral capsid proteins. Binding of the capsid protein of Venezuelan equine encephalitis virus (VEEV) to several types of RNA was used as a model system to test the application of the method. The effects of RNA secondary structures and the significance of electrostatic interaction on binding were identified. This method may have wide application to the Study of RNA-protein interactions. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：155 / 159

页数：5

共 19 条

[1] RNA-protein interactions in spherical viruses [J].