Spatial and Temporal Organization of Chromosome Duplication and Segregation in the Cyanobacterium Synechococcus elongatus PCC 7942

被引：42

作者：

Chen, Anna H. ^{[1
]}

Afonso, Bruno ^{[1
]}

Silver, Pamela A. ^{[1
,2
]}

Savage, David F. ^{[3
,4
]}

机构：

[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02114 USA

[2] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[3] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

PLOS ONE | 2012年 / 7卷 / 10期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

BACTERIAL-DNA REPLICATION; ESCHERICHIA-COLI; CAULOBACTER-CRESCENTUS; POLAR LOCALIZATION; CELL-DIVISION; INITIATION; DYNAMICS; ORIGIN; GROWTH; CYCLE;

D O I：

10.1371/journal.pone.0047837

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The spatial and temporal control of chromosome duplication and segregation is crucial for proper cell division. While this process is well studied in eukaryotic and some prokaryotic organisms, relatively little is known about it in prokaryotic polyploids such as Synechococcus elongatus PCC 7942, which is known to possess one to eight copies of its single chromosome. Using a fluorescent repressor-operator system, S. elongatus chromosomes and chromosome replication forks were tagged and visualized. We found that chromosomal duplication is asynchronous and that the total number of chromosomes is correlated with cell length. Thus, replication is independent of cell cycle and coupled to cell growth. Replication events occur in a spatially random fashion. However, once assembled, replisomes move in a constrained manner. On the other hand, we found that segregation displays a striking spatial organization in some cells. Chromosomes transiently align along the major axis of the cell and timing of alignment was correlated to cell division. This mechanism likely contributes to the non-random segregation of chromosome copies to daughter cells.

引用

页数：10

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