Cell migration and invasion assays

被引：282

作者：

Valster, A

Tran, NL

Nakada, M

Berens, ME

Chan, AY

Symons, M

机构：

[1] Feinstein Inst Med Res N Shore, Manhasset, NY 11030 USA

[2] N Shore Univ Hosp, Dept Surg, Manhasset, NY 11030 USA

[3] Albert Einstein Coll Med, Dept Anat & Struct Biol, Bronx, NY 10461 USA

[4] Translat Genom Res Inst, Neurogenom Div, Phoenix, AZ 85004 USA

来源：

METHODS | 2005年 / 37卷 / 02期

关键词：

migration; invasion; siRNA; Rac; glioma;

D O I：

10.1016/j.ymeth.2005.08.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The processes of cell migration and invasion are integral to embryonic development and the functioning of adult organisms. Deregulation of these processes contributes to numerous diseases. Ras GTPases and in particular members of the Rho subfamily of GTPases play critical roles in cell migration and invasion. Here, we provide a collection of protocols to assay these functions. We describe two cell migration assays. The monolayer wound healing assay is very easy to implement, whereas the microliter-scale migration assay allows examination of cell behavior on defined extracellular matrices. We also describe two methods that allow the quantification of tumor cell invasion, a versatile transwell Matrigel invasion assay and an organotypic assay that examines the invasion of glioma cells through a rat brain slice. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：208 / 215

页数：8

共 47 条

[1] Intravascular origin of metastasis from the proliferation of endothelium-attached tumor cells: a new model for metastasis [J].