Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

被引：88

作者：

Gouveia-Caridade, Carla ^{[1
]}

Pauliukaite, Rasa ^{[1
]}

Brett, Christopher M. A. ^{[1
]}

机构：

[1] Univ Coimbra, Fac Ciencias & Tecnol, Dept Quim, P-3004535 Coimbra, Portugal

来源：

ELECTROCHIMICA ACTA | 2008年 / 53卷 / 23期

关键词：

multi-walled carbon nanotubes; carbon film electrodes; glucose oxidase; alcohol oxidase; eletrochemical biosensor;

D O I：

10.1016/j.electacta.2008.01.040

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Functionalised multi-walled carbon nanotubes (MWCNTs) were cast oil glassy carbon (GC) and carbon film electrodes (CFE), and were characterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified cat-boil film electrodes were then used to develop an alcohol oxidase (AlcOx) biosensor, in which AlcOx-BSA was cross-linked with glutaraldehyde and attached by drop-coating. The experimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at -0.3 V vs. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained was 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：6732 / 6739

页数：8

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