Recent progress in the metabolic engineering of alkaloids in plant systems

被引：71

作者：

Glenn, Weslee S. ^{[1
,2
]}

Runguphan, Weerawat ^{[2
]}

O'Connor, Sarah E. ^{[1
,3
]}

机构：

[1] John Innes Ctr, Dept Biol Chem, Norwich NR4 7UH, Norfolk, England

[2] MIT, Cambridge, MA 02139 USA

[3] Univ E Anglia, Sch Chem, Norwich NR4 7TJ, Norfolk, England

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2013年 / 24卷 / 02期

基金：

英国生物技术与生命科学研究理事会; 美国国家科学基金会; 英国工程与自然科学研究理事会;

关键词：

MICROBIAL-PRODUCTION; CHEMICAL SPACE; ROOT CULTURES; OPIUM POPPY; BIOSYNTHESIS; EXPRESSION; MORPHINE; CYTOCHROME-P450; HALOGENATION; PERIWINKLE;

D O I：

10.1016/j.copbio.2012.08.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant alkaloids have a rich chemical ecology that has been exploited for medicinal purposes for thousands of years. Despite being highly represented within today's pharmacopoeia, relatively little is known about the biosynthesis, regulation and transport of these molecules.. Understanding how nature synthesizes plant alkaloids will enhance our ability to overproduce-that is, to metabolically engineer-these medicinally useful compounds as well as new-to-nature compounds (with potentially improved bioactivity) derived from these natural scaffolds. Recent progress in the metabolic engineering of nitrogen-containing plant natural products-specifically the monoterpene indole alkaloids, the benzylisoquinoline alkaloids and the glucosinolates-was made possible through the characterization of various components in both native and engineered enzymatic pathways. The subsequent reconfiguration and tuning of these biological 'parts' has enabled the production of selected products at increasingly higher titers.

引用

页码：354 / 365

页数：12

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