Optimization of Ultrasound-Assisted Extraction of Single Cell Oil from Mortierella isabellina

被引：9

作者：

Zhou, Cuixia ^{[1
]}

Zhu, Chuanhe ^{[1
]}

Ren, Yuan ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Shandong, Peoples R China

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2013年 / 48卷 / 14期

关键词：

extraction; Mortierella isabellina; single cell oil; ultrasound; GAMMA-LINOLENIC ACID; ALPINA;

D O I：

10.1080/01496395.2013.796562

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this paper, ultrasound-assisted extraction of single cell oil (SCO) from Mortierella isabellina (MI) was investigated using mathematical statistics, such as Plackett-Burman (PB) design, steepest ascent (SA) design, and Box-Behnken (BB) design. The results from response surface methodology (RSM) indicated the hydrochloric acid concentration, ultrasound time, ultrasound temperature, and extraction time are the most significant parameters. The optimum extraction conditions were as follows: ultrasound power 300W, ultrasound time 12.20min, ultrasound temperature 53.42 degrees c, hydrochloric acid 30mmol in each gram of wet fungal mycelia, extraction time 19.45min, extraction solvent (CH3Cl-CH3OH) ratio 2:1. Under the conditions, the extraction rate of SCO from MI was up to 90.63 +/- 1.35%, and the yield of SCO was 109.88 +/- 0.02mg/g (P<0.05) that was 1.23-fold and 1.35-fold the yield of acid-heating method and ultrasound method, respectively.

引用

页码：2188 / 2195

页数：8

共 39 条

[1]

Bao Chuanwen, 2012, Shengwu Jiagong Guocheng, V10, P19, DOI 10.3969/j.issn.1672-3678.2012.02.005

[2]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[3]

Brick E. E., 2000, DEV MED CHILD NEUROL, V42, P174

[4]

Certík M, 2006, FOOD TECHNOL BIOTECH, V44, P75

[5] Biosynthesis and regulation of microbial polyunsaturated fatty acid production [J].

Certik, M ;

Shimizu, S .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 87 (01) :1-14

[6]

[代志凯 Dai Zhikai], 2010, [微生物学通报, Microbiology], V37, P894

[7] Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina [J].

Fakas, Stylianos ;

Papanikolaou, Seraphim ;

Batsos, Athanasios ;

Galiotou-Panayotou, Maria ;

Mallouchos, Athanasios ;

Aggelis, George .

BIOMASS & BIOENERGY, 2009, 33 (04) :573-580

[8] Production of γ-linolenic acid by Cunninghamella echinulata cultivated on glucose and orange peel [J].

Gema, H ;

Kavadia, A ;

Dimou, D ;

Tsagou, V ;

Komaitis, M ;

Aggelis, G .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 58 (03) :303-307

[9] Ultrasound-assisted extraction of pomegranate seed oil - Kinetic modeling [J].

Goula, Athanasia M. .

JOURNAL OF FOOD ENGINEERING, 2013, 117 (04) :492-498

[10]

Han Y. X., 2011, J FARM PRODUCTS P, V232, P51

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