X-ray Free Electron Lasers Motivate Bioanalytical Characterization of Protein Nanocrystals: Serial Femtosecond Crystallography

被引:25
作者
Bogan, Michael J. [1 ]
机构
[1] Stanford PULSE Inst, SLAC Natl Accelerator Lab, Menlo Pk, CA 94025 USA
关键词
MASS-SPECTROMETRY; PHOTOSYSTEM-II; DIFFRACTION; TIME; NANOPARTICLES; RESOLUTION; CRYSTALS; DAMAGE; CELLS;
D O I
10.1021/ac303716r
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Atomic resolution structures of large biomacromolecular complexes can now be recorded at room temperature from crystals with submicrometer dimensions using intense femtosecond pulses delivered by the world's largest and most powerful X-ray machine, a laser called the Linac Coherent Light Source. Abundant opportunities exist for the bioanalytical sciences to help extend this revolutionary advance in structural biology to the ultimate goal of recording molecular-movies of noncrystalline biomacromolecules. This Feature will introduce the concept of serial femtosecond crystallography to the nonexpert, briefly review progress to date, and highlight some potential contributions from the analytical sciences.
引用
收藏
页码:3464 / 3471
页数:8
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