Potato E3 ubiquitin ligase PUB27 negatively regulates drought tolerance by mediating stomatal movement

被引:10
|
作者
Tang, Xun [1 ,2 ]
Ghimire, Shantwana [1 ,3 ]
Liu, Weigang [1 ,3 ]
Fu, Xue [1 ,2 ]
Zhang, Huanhuan [1 ,2 ]
Zhang, Ning [1 ,2 ]
Si, Huaijun [1 ,2 ]
机构
[1] Gansu Agr Univ, Gansu Prov Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China
[2] Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China
[3] Gansu Agr Univ, Coll Agron, Lanzhou 730070, Peoples R China
基金
中国国家自然科学基金;
关键词
StPUB27; Drought stress; Genetic transformation; Stomatal movement; Solanum tuberosum; STRESS TOLERANCE; ABSCISIC-ACID; RESPONSES; PLANTS;
D O I
10.1016/j.plaphy.2020.07.026
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The ubiquitin-proteasome system (UPS) is one of the main ways of eukaryotic protein degradation and post-translational modification. It has proven as an essential process for plants to respond to abiotic stresses. Plant U-box (PUB) protein acts as a ubiquitin ligase, which recognizes and ubiquitinates the target proteins. Many PUBs have been involved in water stress in Arabidopsis and rice, but similar comprehensive studies in potato remained limited. In this study, the overexpressed and interfered transgenic potato plants of StPUB27 were obtained and their performances were evaluated under osmotic stress. The result showed that overexpression of StPUB27 accelerated the dehydration of detached leaves companied with greater stomatal conductance, while the down-regulated StPUB27 expression by RNA interference (RNAi) showed a smaller stomatal conductance and a lower rate of water loss in detached leaves, thus showing higher tolerance to osmotic stress. In addition, no significant changes in the proline content were observed between StPUB27 overexpressed and RNAi potato plants. The result demonstrated that potato E3 ubiquitin ligase PUB27 may negatively regulate drought tolerance by mediating stomatal conductance.
引用
收藏
页码:557 / 563
页数:7
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