Relationship between the degree of antioxidant protection and the level of malondialdehyde in high-performance Polish Holstein-Friesian cows in peak of lactation

被引:38
作者
Kapusta, Aleksandra [1 ]
Kuczynska, Beata [1 ]
Puppel, Kamila [1 ]
机构
[1] Warsaw Univ Life Sci, Anim Breeding & Prod Dept, Cattle Breeding Div, Warsaw, Poland
来源
PLOS ONE | 2018年 / 13卷 / 03期
关键词
CHOLESTEROL OXIDATION-PRODUCTS; DAIRY-COWS; STRESS; MILK; STABILITY; MALONALDEHYDE; CAROTENOIDS; PLASMA; PERIOD; STAGE;
D O I
10.1371/journal.pone.0193512
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Lipid peroxidation can be described as a process under which free radicals attack carbon double bonds of omega-3 and omega-6 fatty acids. Whereas the end products of this process are reactive aldehydes, such as malondialdehyde ( MDA). Lipid peroxidation leads to adverse changes in the nutritional value of milk; therefore, higher degree of antioxidant protection ( DAP) ensures higher stability of dairy products by effecting their high antioxidative potential. Therefore, the purpose of this study was to demonstrate the relationship between the DAP and the level of MDA in high-performance Polish Holstein-Friesian cows in peak of lactation. Sixty-three Polish Holstein-Friesian cows were selected to the experiment according to: parity ( all in the 2nd lactation), phase of lactation ( peak of lactation), cytological quality of milk ( somatic cell count < 150 thousand/ml) and without diagnosed metabolic diseases. The data obtained were analyzed statistically by two-way ANOVA, and Tukey's post-hoc test. After analysis of performance the cows were divided into 3 groups ( twenty one cows in each group) based on milk yield and MDA concentration. The study revealed a significant effect of the lactation performance of cows on MDA levels in milk ( P <= 0.01). The highest concentration of MDA ( 61.137 nM/mL) was shown in milk of cows yielding between 50.00 and 55.80 kg/day. The highest concentration of fat was found in milk in which the MDA level ranged from 48 to 86 nM/mL. Whereas, the inverse relationship was demonstrated in case of protein concentration. The highest level of protein was found in cows with MDA levels in the range of 18-28 nM/ mL ( P <= 0.01). The lowest MDA level ( in the range of 18-28 nM/mL) was associated with the highest concentration of vitamin E, beta-carotene, total antioxidant status ( TAS) and DAP, measured in both milk and plasma. The obtained results show that lipid peroxidation leads to adverse changes in the nutritional value of milk; the highest DAP ( 7.89 x 10(-3)) was found in the cows with the lowest MDA concentration in milk.
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页数:13
相关论文
共 41 条
[1]  
[Anonymous], 5509 ISO
[2]  
Armonk N.I., 2017, IBM SPSS Statistics for Windows
[3]  
Association of Official Analytical Chemists, 1990, OFFICIAL METHODS ANA, V1
[4]   Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal [J].
Ayala, Antonio ;
Munoz, Mario F. ;
Argueelles, Sandro .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2014, 2014
[5]   ANTIOXIDANT MICRONUTRIENTS AND IMMUNE-RESPONSES [J].
BENDICH, A .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1990, 587 :168-180
[6]   Plasma malonaldehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows [J].
Castillo, C ;
Hernández, J ;
Valverde, I ;
Pereira, V ;
Sotillo, J ;
Alonso, ML ;
Benedito, JL .
RESEARCH IN VETERINARY SCIENCE, 2006, 80 (02) :133-139
[7]   Biomarkers of oxidative stress in ruminant medicine [J].
Celi, Pietro .
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, 2011, 33 (02) :233-240
[8]  
Chew BP, 1993, J ANIM SCI, P710
[9]  
Diplock AT, 1981, SELENIUM BIOL MED, P303
[10]  
ESTERBAUER H, 1990, METHOD ENZYMOL, V186, P407