Genotypic and Phenotypic Evaluation of the Evolution of High-Level Daptomycin Nonsusceptibility in Vancomycin-Resistant Enterococcus faecium

被引：50

作者：

Humphries, Romney M. ^{[1
]}

Kelesidis, Theodoros ^{[2
]}

Tewhey, Ryan ^{[3
]}

Rose, Warren E. ^{[4
]}

Schork, Nicholas ^{[3
,5
]}

Nizet, Victor ^{[6
]}

Sakoulas, George ^{[6
]}

机构：

[1] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Div Infect Dis, Dept Med, Los Angeles, CA 90095 USA

[3] Scripps Translat Sci Inst, La Jolla, CA USA

[4] Univ Wisconsin, Madison Sch Pharm, Madison, WI USA

[5] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA

[6] Univ Calif San Diego, Sch Med, Div Pediat Pharmacol & Drug Discovery, La Jolla, CA 92093 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2012年 / 56卷 / 11期

关键词：

STAPHYLOCOCCUS-AUREUS; DE-NOVO; BACTERIOCINS; INFECTIONS; MECHANISMS; ALIGNMENT; TARGET;

D O I：

10.1128/AAC.01318-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Whole-genome sequencing and cell membrane studies of three clonal Enterococcus faecium strains with daptomycin MICs of 4, 32, and 192 mu g/ml were performed, revealing nonsynonymous single nucleotide variants in eight open reading frames, including those predicted to encode a phosphoenolpyruvate-dependent, mannose-specific phosphotransferase system, cardiolipin synthetase, and EzrA. Membrane studies revealed a higher net surface charge among the daptomycin-nonsusceptible isolates and increased septum formation in the isolate with a daptomycin MIC of 192 mu g/ml.

引用

页码：6051 / 6053

页数：3

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