Protective Effect of Genistein on Lipopolysaccharide/D-Galactosamine-Induced Hepatic Failure in Mice

被引：19

作者：

Lin, Xing ^{[1
]}

Zhang, Shijun ^{[1
]}

Huang, Renbin ^{[1
]}

Wei, Ling ^{[1
]}

Liang, Chunhong ^{[1
]}

Chen, Yongxing ^{[1
]}

Lv, Shujuan ^{[1
]}

Liang, Shuang ^{[2
]}

Wu, Xiaoyan ^{[2
]}

Huang, Quanfang ^{[2
]}

机构：

[1] Guangxi Med Univ, Nanning 530021, Peoples R China

[2] Guangxi Univ Chinese Med, Affiliated Hosp 1, Nanning 530023, Peoples R China

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2014年 / 37卷 / 04期

基金：

中国国家自然科学基金;

关键词：

genistein; Hydrocotyle sibthorpioides; lipopolysaccharide; D-galactosamine; acute hepatic failure; TUMOR-NECROSIS-FACTOR; HEME OXYGENASE; CARBON-MONOXIDE; UP-REGULATION; CYTOCHROME-C; LIVER-INJURY; KAPPA-B; EXPRESSION; APOPTOSIS; RELEASE;

D O I：

10.1248/bpb.b13-00908

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

This study examined the effect of genistein from Hydrocotyle sibthorpioides on lipopolysaccharide (LPS)/D-galactosamine (D-GalN)-induced acute hepatic failure. Compared to the model control, genistein treatment significantly protected against LPS/D-GalN-induced liver injury, as evidenced by the decrease in serum alanine and aspartate aminotransferases activities and the attenuation of histopathological changes. Furthermore, genistein alleviated the pro-inflammatory cytokines including tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO)/inducible nitric oxide synthase (iNOS) by inhibiting nuclear factor-kappa B (NF-kappa B) activity. Genistein attenuated the elevated level of caspases-3, while augmented the expression of Bcl-2. In addition, LPS/D-GalN induced significant increase of heme oxygenase (HO), carbon monoxide and bilirubin levels and these alterations were augmented by genistein treatment. In conclusion, the protective effect of genistein on LPS/D-GalN-induced liver damage was mainly due to its ability to block NF-kappa B signaling pathway for anti-inflammation response, attenuate hepatocellular apoptosis and increase HO level. These findings suggest that genistein can be considered as a potential agent for preventing acute hepatic failure.

引用

页码：625 / 632

页数：8

共 31 条

[1] APPARENT HYDROXYL RADICAL PRODUCTION BY PEROXYNITRITE - IMPLICATIONS FOR ENDOTHELIAL INJURY FROM NITRIC-OXIDE AND SUPEROXIDE [J].