Genetic relatedness of methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolated from a dog and the dog owner

被引:38
作者
Soedarmanto, I. [1 ,2 ]
Kanbar, T. [2 ]
Uelbegi-Mohyla, H. [2 ]
Hijazin, M. [2 ]
Alber, J. [2 ]
Laemmler, C. [2 ]
Akineden, Oe. [3 ]
Weiss, R. [4 ]
Moritz, A. [5 ]
Zschoeck, M. [6 ]
机构
[1] Univ Gadjah Mada, Fac Vet Med, Dept Vet Internal Med, Yogyakarta 55281, Indonesia
[2] Univ Giessen, Inst Pharmakol & Toxikol, D-35392 Giessen, Germany
[3] Univ Giessen, Inst Tierarztliche Nahrungsmittelkunde, Milchwissensch, D-35390 Giessen, Germany
[4] Univ Giessen, Inst Hyg & Infekt Krankheiten Tiere, D-35392 Giessen, Germany
[5] Univ Giessen, Klin Kleintiere, Innere Med, D-35392 Giessen, Germany
[6] Landesbetrieb Hess Landeslab, D-35396 Giessen, Germany
关键词
Staphylococcus pseudintermedius; Dog; Dog owner; Methicillin resistant; Genetic relatedness; MOLECULAR CHARACTERIZATION; PET ANIMALS; INTERMEDIUS; TRANSMISSION; HUMANS; AUREUS; PREVALENCE; EMERGENCE; SKIN;
D O I
10.1016/j.rvsc.2011.01.027
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In the present study four methicillin-resistant Staphylococcus pseudinterrnedius (MRSP) strains isolated from a dog (n = 3) and the anterior nares of the dog owner (n = 1) were investigated by conventional and molecular methods. The species identity of the four S. pseudintermedius strains was confirmed by conventional methods, by PCR mediated amplification of S. intermedius/S. pseudintermedius specific segments of thermonuclease encoding gene nuc and by restriction fragment length polymorphism analysis of phosphoacetyltransferase encoding gene pta. Investigation of the four S. pseudintermedius for toxino-genic potential revealed that all four strains were positive for the exfoliative toxin encoding gene siet and the leukotoxin encoding genes lukS, lukF. The oxacillin and penicillin resistance of the four S. pseudintermedius strains could be determined by cultivation of the strains on oxacillin resistant screening agar base, ChromID MRSA Agar and Brilliance MRSA Agar and by multiplex PCR detecting the resistance genes mecA and blaZ. The genetic relatedness of the strains was studied by macrorestriction analysis of their chromosomal DNA using pulsed field gel electrophoresis (PFGE). According to PFGE all four S. pseudintermedius strains represent an identical bacterial clone indicating a cross transmission between the dog and the dog owner. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:E25 / E27
页数:3
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