Increased SUMO-1 expression in the unilateral rotenone-lesioned mouse model of Parkinson's disease

被引：26

作者：

Weetman, J. ^{[1
]}

Wong, M. B. ^{[1
]}

Sharry, S. ^{[1
]}

Rcom-H'cheo-Gauthier, A. ^{[1
]}

Gai, W. P. ^{[2
]}

Meedeniya, A. ^{[1
]}

Pountney, D. L. ^{[1
]}

机构：

[1] Griffith Univ, Sch Med Sci, Griffith, Qld 4222, Australia

[2] Flinders Univ S Australia, Ctr Neurosci, Adelaide, SA 5001, Australia

来源：

NEUROSCIENCE LETTERS | 2013年 / 544卷

基金：

澳大利亚研究理事会; 澳大利亚国家健康与医学研究理事会;

关键词：

alpha-Synuclein; SUMO; Rotenone; Parkinson's disease; UBIQUITIN; AGGREGATION; INCLUSIONS; MARKS;

D O I：

10.1016/j.neulet.2013.03.057

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Parkinson's disease (PD) is a neurodegenerative disease resulting from progressive loss of dopaminergic nigrostriatal neurons.alpha-Synuclein protein conformational changes, resulting in cytotoxic/aggregated proteins, have been linked to PD pathogenesis. We investigated a unilateral rotenone-lesioned mouse PD model. Unilateral lesion of the medial forebrain bundle for two groups of male C57 black mice (n=5); adult (6-12 months) group and aged (1.75-2 years) group, was via stereotactic rotenone injection. After 2 weeks post-lesion, phenotypic Parkinsonian symptoms, resting tremor, postural instability, left-handed bias, ipsiversive rotation and bradykinesia were observed and were more severe in the aged group. We investigated protein expression profiles of the post-translational modifier, SUMO-1, and alpha-synuclein between the treated and control hemisphere, and between adult and aged groups. Western analysis of the brain homogenates indicated that there were statistically significant (p < 0.05) increases in several specific molecular weight species (ranging 12-190 kDa) of both SUMO-1 (0.75-4.3-fold increased) and alpha-synuclein (1.6-19-fold increase) in the lesioned compared to un-lesioned hemisphere, with the adult mice showing proportionately greater increases in SUMO-1 than the aged group. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

引用

页码：119 / 124

页数：6

共 28 条

[1] PROTEIN SUMOYLATION IN NEUROPATHOLOGICAL CONDITIONS [J].

Anderson, Dina B. ;

Wilkinson, Kevin A. ;

Henley, Jeremy M. .

DRUG NEWS & PERSPECTIVES, 2009, 22 (05) :255-265

[2]

Archibald N., 2008, Medicine, V36, P630, DOI [10.1016/j.mpmed.2008.09.004, DOI 10.1016/J.MPMED.2008.09.004]

[3] Chronic systemic pesticide exposure reproduces features of Parkinson's disease [J].

Betarbet, R ;

Sherer, TB ;

MacKenzie, G ;

Garcia-Osuna, M ;

Panov, AV ;

Greenamyre, JT .

NATURE NEUROSCIENCE, 2000, 3 (12) :1301-1306

[4] Stanley Fahn Lecture 2005:: The staging procedure for the inclusion body pathology associated with sporadic Parkinson's disease reconsidered [J].

Braak, Heiko ;

Bohl, Juergen R. ;

Muller, Christian M. ;

Rub, Udo ;

de Vos, Rob A. I. ;

Del Tredici, Kelly .

MOVEMENT DISORDERS, 2006, 21 (12) :2042-2051

[5]

Brown R.C., 2005, ENV HLTH PERSPECT, V113, P5

[6] Further evidence that interactions between CYP2D6 and pesticide exposure increase risk for Parkinson's disease [J].

Deng, YF ;

Newman, B ;

Dunne, MP ;

Silburn, PA ;

Mellick, GD .

ANNALS OF NEUROLOGY, 2004, 55 (06) :897-897

[7] Protein folding and misfolding [J].

Dobson, CM .

NATURE, 2003, 426 (6968) :884-890

[8] Projected number of people with Parkinson disease in the most populous nations, 2005 through 2030 [J].

Dorsey, E. R. ;

Constantinescu, R. ;

Thompson, J. P. ;

Biglan, K. M. ;

Holloway, R. G. ;

Kieburtz, K. ;

Marshall, F. J. ;

Ravina, B. M. ;

Schifitto, G. ;

Siderowf, A. ;

Tanner, C. M. .

NEUROLOGY, 2007, 68 (05) :384-386

[9] Small ubiquitin-like modifier (SUMO) modification of natively unfolded proteins tau and α-synuclein [J].

Dorval, V ;

Fraser, PE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (15) :9919-9924

[10] Inhibitors of NADH-ubiquinone reductase: an overview [J].

Esposti, MD .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1364 (02) :222-235

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