Biocatalytic preparation of chiral epichlorohydrins using recombinant Pichia pastoris expressing epoxide hydrolase of Rhodotorula glutinis

被引：39

作者：

Kim, HS

Lee, JH

Park, S

Lee, EY ^{[1
]}

机构：

[1] Kyungsung Univ, Dept Food Sci & Technol, Pusan 608736, South Korea

[2] Silla Univ, Dept Biosci & Biotechnol, Pusan 617736, South Korea

[3] Pusan Natl Univ, Dept Chem & Biochem Engn, Pusan 609735, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2004年 / 9卷 / 01期

关键词：

enantioselective hydrolysis; epichlorohydrin; epoxide hydrolase; Rhodotorula glutinis; Pichia pastoris;

D O I：

10.1007/BF02949324

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The use of enantioselective hydrolysis for preparing chiral epichlorohydrins was investigated using recombinant Pichia pastoris with the enantioselective epoxide hydrolase of Rhodotorula glutinis. The rate of the recombinant epoxide hydrolase-catalyzed enantioselective hydrolysis of racemic epichlorohydrins was enhanced by the addition of 5% (v/v) Tween 20. Enantiopure (R)-epichlorohydrins with an enantiopurity of 100% ee and a yield of 26% were obtained within 5 min from 50 mM racemates.

引用

页码：62 / 64

页数：3

共 15 条

[1] Biocatalytic preparation of chiral epichlorohydrins using recombinantPichia pastoris expressing epoxide hydrolase ofRhodotorula glutinis
Hee Sook Kim
Jae-Hwa Lee
Sunghoon Park
Eun Yeol Lee
Biotechnology and Bioprocess Engineering, 2004, 9 : 62 - 64
[2] Production of (S)-styrene oxide by recombinant Pichia pastoris containing epoxide hydrolase from Rhodotorula glutinis
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Yoo, SS
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