Development of a polymerase chain reaction applicable to rapid and sensitive detection of Clonorchis sinensis eggs in human stool samples

被引：16

作者：

Cho, Pyo Yun ^{[1
,2
]}

Na, Byoung-Kuk ^{[3
,4
]}

Choi, Kyung Mi ^{[5
]}

Kim, Jin Su ^{[1
,2
]}

Cho, Shin-Hyeong ^{[6
]}

Lee, Won-Ja ^{[6
]}

Lim, Sung-Bin ^{[7
]}

Cha, Seok Ho ^{[1
,2
]}

Park, Yun-Kyu ^{[1
,2
]}

Pak, Jhang Ho ^{[8
]}

Lee, Hyeong-Woo ^{[9
]}

Hong, Sung-Jong ^{[10
]}

Kim, Tong-Soo ^{[1
,2
]}

机构：

[1] Inha Univ, Sch Med, Dept Parasitol, Inchon 400712, South Korea

[2] Inha Univ, Sch Med, Inha Res Inst Med Sci, Inchon 400712, South Korea

[3] Gyeongsang Natl Univ, Sch Med, Dept Parasitol, Jinju 660751, South Korea

[4] Gyeongsang Natl Univ, Sch Med, Inst Hlth Sci, Jinju 660751, South Korea

[5] Korea Inst Sci & Technol, Biomed Res Inst, Ctr Theragnosis, Seoul 136791, South Korea

[6] Natl Inst Hlth, Div Malaria & Parasit Dis, Osong 363951, South Korea

[7] Dongguk Univ, Dept Life Sci, Seoul 100715, South Korea

[8] Univ Ulsan, Coll Med, Asan Med Ctr, Asan Inst Life Sci, Seoul 138736, South Korea

[9] Univ Florida, Coll Med, Dept Pathol, Gainesville, FL 32610 USA

[10] Chung Ang Univ, Coll Med, Dept Med Environm Biol, Seoul 156756, South Korea

来源：

PATHOGENS AND GLOBAL HEALTH | 2013年 / 107卷 / 05期

关键词：

Clonorchis sinensis; Egg; PCR diagnosis; Retrotransposon; OPISTHORCHIS-VIVERRINI; PCR ASSAY; SERODIAGNOSIS; DIAGNOSIS; DNA; SPECIMENS;

D O I：

10.1179/2047773213Y.0000000099

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Microscopic examination of eggs of parasitic helminths in stool samples has been the most widely used classical diagnostic method for infections, but tiny and low numbers of eggs in stool samples often hamper diagnosis of helminthic infections with classical microscopic examination. Moreover, it is also difficult to differentiate parasite eggs by the classical method, if they have similar morphological characteristics. In this study, we developed a rapid and sensitive polymerase chain reaction (PCR)-based molecular diagnostic method for detection of Clonorchis sinensis eggs in stool samples. Nine primers were designed based on the long-terminal repeat (LTR) of C. sinensis retrotransposon1 (CsRn1) gene, and seven PCR primer sets were paired. Polymerase chain reaction with each primer pair produced specific amplicons for C. sinensis, but not for other trematodes including Metagonimus yokogawai and Paragonimus westermani. Particularly, three primer sets were able to detect 10 C. sinensis eggs and were applicable to amplify specific amplicons from DNA samples purified from stool of C. sinensis-infected patients. This PCR method could be useful for diagnosis of C. sinensis infections in human stool samples with a high level of specificity and sensitivity.

引用

页码：253 / 259

页数：7

共 33 条

[1] CsRn1, a novel active retrotransposon in a parasitic trematode, Clonorchis sinensis, discloses a new phylogenetic clade of Ty3/gypsy-like LTR retrotransposons [J].