Identification of a newly isolated erythritol-producing yeast and cloning of its erythritol reductase genes

被引：21

作者：

Deng, Huihui ^{[1
]}

Han, Ye ^{[1
]}

Liu, Yuanyuan ^{[1
]}

Jia, Wei ^{[1
]}

Zhou, Zhijiang ^{[1
]}

机构：

[1] Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2012年 / 39卷 / 11期

关键词：

Moniliella; Identification; Erythritol; Erythritol reductase gene; FED-BATCH CULTURES; TOMENTOSA-VAR-POLLINIS; ERYTHROSE REDUCTASE; CANDIDA-MAGNOLIAE; TORULA SP; ENGINEERING ASPECTS; OSMOPHILIC MUTANT; AUREOBASIDIUM SP; SUGAR ALCOHOLS; MONILIELLA;

D O I：

10.1007/s10295-012-1162-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new erythritol-producing yeast (strain BH010) was isolated in this study. Analysis of the D1/D2 domain of the 26S rDNA sequence, the ITS/5.8S rDNA sequence, and the 18S rDNA sequence allowed the taxonomic position of strain BH010 to be discussed and it was identified and named Moniliella sp. BH010. Physiological characteristics were described. Scanning electron micrography clearly indicated that the cells were cylindrical to elliptical with an average size of 5 x 10 mu m when growing in liquid medium, and that pseudohyphae and blastoconidia were observed when cultivated in agar plates. The erythritol reductase genes were cloned, sequenced, and analyzed. BLAST analysis and multiple sequence alignment demonstrated that erythritol reductase genes of Moniliella sp. BH010 shared very high homology with that of Trichosporonoides megachiliensis SNG-42 except for the presence of introns. The deduced amino acid sequences showed high homology to the aldo-keto reductase superfamily.

引用

页码：1663 / 1672

页数：10

共 53 条

[1]

Anderson IC, 2003, ENVIRON MICROBIOL, V5, P1121, DOI [10.1046/j.1462-2920.2003.00522.x, 10.1046/j.1462-2920.2003.00383.x]

[2]

BARNETT J.A., 1983, YEASTS CHARACTERISTI

[3] CHROMATOGRAPHIC FRACTIONATION OF CANE BLACKSTRAP MOLASSES AND OF ITS FERMENTATION RESIDUE [J].

BINKLEY, WW ;

WOLFROM, ML .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1950, 72 (10) :4778-4782

[4] Engineering aspects of the production of sugar alcohols with the osmophilic yeast Moniliella tomentosa var pollinis Part 2.: Batch and fed-batch operation in bubble column and airlift tower loop if reactors [J].

Burschäpers, J ;

Schustolla, D ;

Schügerl, K ;

Röper, H ;

de Troostembergh, JC .

PROCESS BIOCHEMISTRY, 2002, 38 (04) :559-570

[5] Engineering aspects of the production of sugar alcohols with the osmophilic yeast Moniliella tomentosa var pollinis.: Part I.: Batch and fed-batch operation in stirred tank [J].

Burschäpers, J ;

Schustolla, D ;

Schügerl, K ;

Röper, H ;

de Troostembergh, JC .

PROCESS BIOCHEMISTRY, 2002, 38 (04) :497-506

[6]

Cho C. H., 1999, FOOD SCI BIOTECHNOL, V8, P73

[7] Erythritol is a sweet antioxidant [J].

den Hartog, Gertjan J. M. ;

Boots, Agnes W. ;

Adam-Perrot, Aline ;

Brouns, Fred ;

Verkooijen, Inge W. C. M. ;

Weseler, Antje R. ;

Haenen, Guido R. M. M. ;

Bast, Aalt .

NUTRITION, 2010, 26 (04) :449-458

[8] EVOLUTIONARY AFFINITIES OF HETEROBASIDIOMYCETOUS YEASTS ESTIMATED FROM 18S AND 25S RIBOSOMAL-RNA SEQUENCE DIVERGENCE [J].

GUEHO, E ;

KURTZMAN, CP ;

PETERSON, SW .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1989, 12 (03) :230-236

[9] A COMPILATION OF LARGE SUBUNIT RNA SEQUENCES PRESENTED IN A STRUCTURAL FORMAT [J].

GUTELL, RR ;

FOX, GE .

NUCLEIC ACIDS RESEARCH, 1988, 16 :R175-R269

[10] ERYTHRITOL PRODUCTION BY YEASTLIKE FUNGUS [J].

HAJNY, GJ ;

GARVER, JC ;

SMITH, JH .

APPLIED MICROBIOLOGY, 1964, 12 (03) :240-&

← 1 2 3 4 5 6 →