Studies on the electrochemistry of rutin and its interaction with bovine serum albumin using a glassy carbon electrode modified with carbon-coated nickel nanoparticles

被引：15

作者：

Duan, Liansheng ^{[1
,2
,3
]}

Yang, Lijun ^{[1
,2
]}

Xiong, Huayu ^{[1
,2
]}

Zhang, Xiuhua ^{[1
,2
]}

Wang, Shengfu ^{[1
,2
]}

机构：

[1] Hubei Univ, Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 430062, Peoples R China

[2] Hubei Univ, Coll Chem & Chem Engn, Wuhan 430062, Peoples R China

[3] Hubei Univ Educ, Dept Chem & Life Sci, Wuhan 430205, Peoples R China

来源：

MICROCHIMICA ACTA | 2013年 / 180卷 / 5-6期

基金：

中国国家自然科学基金; 湖北省教育厅重点项目;

关键词：

Rutin; Bovine serum albumin; Carbon-coated nickel nanoparticles; Interaction; CAPILLARY-ELECTROPHORESIS; AMPEROMETRIC DETECTION; ARC-DISCHARGE; CYCLODEXTRIN; SENSOR;

D O I：

10.1007/s00604-012-0931-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report on a glassy carbon electrode modified with carbon-coated nickel nanoparticles (C-Ni/GCE) that can be used to study the electrochemical properties of rutin and its interaction with bovine serum albumin (BSA) via cyclic voltammetry and differential pulse voltammetry. The effects of pH value, accumulation potential, accumulation time and reaction time were optimized. A pair of reversible peaks is found in the potential range of 0 to around 0.6 V at pH 5.0. Two linear response ranges (with different slopes) are found, one in the 2 to 210 nM concentration range, the other between 0.21 and 1.72 mu M. The detection limit is as low as 0.6 nM. On addition of BSA to the rutin solution, a decrease of the current is observed that is proportional to the concentration of BSA. The binding constant and stoichiometric ratio were calculated.

引用

页码：355 / 361

页数：7

共 32 条

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