Circular Sponge against miR-21 Enhances the Antitumor Activity of Doxorubicin against Breast Cancer Cells

被引:3
作者
Rama, Ana R. [1 ,2 ]
Lara, Patricia [2 ]
Mesas, Cristina [2 ,3 ,4 ]
Quinonero, Francisco [2 ,3 ,4 ]
Velez, Celia [2 ,3 ,4 ]
Melguizo, Consolacion [2 ,3 ,4 ]
Prados, Jose [2 ,3 ,4 ]
机构
[1] Univ Jaen, Dept Hlth Sci, Jaen 23071, Spain
[2] Univ Granada, Ctr Biomed Res CIBM, Inst Biopathol & Regenerat Med IBIMER, Granada 18100, Spain
[3] Inst Biosanit Res Granada Ibs GRANADA, Granada 18014, Spain
[4] Univ Granada, Fac Med, Dept Anat & Embryol, Granada 18016, Spain
关键词
miRNA; circular sponges; miR-21; doxorubicin; breast cancer; MULTIDRUG-RESISTANCE; DOWN-REGULATION; MESENCHYMAL TRANSITION; PROLIFERATION; MIRNA-21; TRANSPORTER; INHIBITION; EXPRESSION; MICRORNAS; MIGRATION;
D O I
10.3390/ijms232314803
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Breast cancer is the most common type of cancer in women, with chemotherapy being the main strategy. However, its effectiveness is reduced by drug resistance mechanisms. miR-21 is upregulated in breast cancer that has been linked to drug resistance and carcinogenic processes. Our aim was to capture miR-21 with a circular sponge (Circ-21) and thus inhibit the carcinogenic processes and drug resistance mechanisms in which it participates. Proliferation, migration, colony formation, cell cycle, and poly [ADP-ribose] polymerase 1 (PARP-1) and vascular endothelial growth factor (VEGF) detection assays were performed with MCF7 breast cancer cells and MCF10A non-tumor cells. In addition, doxorubicin resistance tests and detection of drug resistance gene expression were performed in MCF7 cells. Reduction in proliferation, as well as migration and colony formation, increased PARP-1 expression, inhibition of VEGF expression and cell cycle arrest in G2/M phase were displayed in the Circ-21 MCF7, which were not observed in the MCF10A cells. Furthermore, in the MCF7 cells, the Circ-21 enhanced the antitumor activity of doxorubicin and decreased the expression of resistance genes: ABCA1, ABCC4, and ABCC5. Based on these results, the use of Circ-21 can be considered a first step for the establishment of an effective gene therapy in the treatment of breast cancer.
引用
收藏
页数:16
相关论文
共 50 条
  • [41] Cerium oxide nanoparticles exert antitumor effects and enhance paclitaxel toxicity and activity against breast cancer cells
    Sekeroglu, Zulal Atli
    Sekeroglu, Vedat
    Aydin, Birsen
    Yedier, Seval Kontas
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2023, 111 (03) : 579 - 589
  • [42] Androgens downregulate miR-21 expression in breast cancer cells underlining the protective role of androgen receptor
    Casaburi, Ivan
    Cesario, Maria Grazia
    Dona, Ada
    Rizza, Pietro
    Aquila, Saveria
    Avena, Paola
    Lanzino, Marilena
    Pellegrino, Michele
    Vivacqua, Adele
    Tucci, Paola
    Morelli, Catia
    Ando, Sebastiano
    Sisci, Diego
    ONCOTARGET, 2016, 7 (11) : 12651 - 12661
  • [43] Circulating miR-21 is Down-Regulated Following Adjuvant Chemotherapy in Breast Cancer Patients
    Kazemi Nezhad, S. R.
    Baghbani, H.
    Sasan, H.
    Hajjari, M. R.
    RUSSIAN JOURNAL OF GENETICS, 2020, 56 (01) : 103 - 107
  • [44] Human cancer cells suppress behaviors of endothelial progenitor cells through miR-21 targeting IL6R
    Wang, Wenbin
    Yuan, Xiao
    Xu, Aman
    Zhu, Xingyang
    Zhan, Yanqing
    Wang, Shuanhu
    Liu, Mulin
    MICROVASCULAR RESEARCH, 2018, 120 : 21 - 28
  • [45] miR-21 inhibitor facilitates the anticancer activity of doxorubicin loaded nanometer in melanoma
    Wei, Xiaoyong
    You, Xiaolong
    Zhang, Jianlong
    Zhou, Cuncai
    ONCOLOGY REPORTS, 2019, 42 (01) : 414 - 424
  • [46] The anti-metastatic activity of collagenase-2 in breast cancer cells is mediated by a signaling pathway involving decorin and miR-21
    Soria-Valles, C.
    Gutierrez-Fernandez, A.
    Guiu, M.
    Mari, B.
    Fueyo, A.
    Gomis, R. R.
    Lopez-Otin, C.
    ONCOGENE, 2014, 33 (23) : 3054 - 3063
  • [47] Antitumor activity of terpenoids against classical and atypical multidrug resistant cancer cells
    Lage, H.
    Duarte, N.
    Coburger, C.
    Hilgeroth, A.
    Ferreira, M. J. U.
    PHYTOMEDICINE, 2010, 17 (06) : 441 - 448
  • [48] Discovery of a synthetic taiwaniaquinoid with potent in vitro and in vivo antitumor activity against breast cancer cells
    Mut-Salud, Nuria
    Guardia, Juan J.
    Fernandez, Antonio
    Blancas, Isabel
    Zentar, Houda
    Garrido, Jose M.
    Alvarez-Manzaneda, Enrique
    Chahboun, Rachid
    Rodriguez-Serrano, Fernando
    BIOMEDICINE & PHARMACOTHERAPY, 2023, 168
  • [49] Salinomycin enhances doxorubicin-induced cytotoxicity in multidrug resistant MCF-7/MDR human breast cancer cells via decreased efflux of doxorubicin
    Kim, Kwang-Youn
    Kim, Sang-Hun
    Yu, Sun-Nyoung
    Park, Suel-Ki
    Choi, Hyeun-Deok
    Yu, Hak-Sun
    Ji, Jae-Hoon
    Seo, Young-Kyo
    Ahn, Soon-Cheol
    MOLECULAR MEDICINE REPORTS, 2015, 12 (02) : 1898 - 1904
  • [50] MiR-21 protected against diabetic cardiomyopathy induced diastolic dysfunction by targeting gelsolin
    Dai, Beibei
    Li, Huaping
    Fan, Jiahui
    Zhao, Yanru
    Yin, Zhongwei
    Nie, Xiang
    Wang, Dao Wen
    Chen, Chen
    CARDIOVASCULAR DIABETOLOGY, 2018, 17