Discovery and profiling of novel and conserved microRNAs during flower development in Carya cathayensis via deep sequencing

被引：57

作者：

Wang, Zheng Jia ^{[1
,2
]}

Huang, Jian Qin ^{[1
,2
]}

Huang, You Jun ^{[1
,2
]}

Li, Zheng ^{[1
,2
]}

Zheng, Bing Song ^{[1
,2
]}

机构：

[1] Zhejiang A&F Univ, Sch Forestry & Biotechnol, Hangzhou 311300, Zhejiang, Peoples R China

[2] Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Nurturing Stn, Hangzhou 311300, Zhejiang, Peoples R China

来源：

PLANTA | 2012年 / 236卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Flower development; Hickory; MicroRNA; Solexa sequencing; STRESS-RESPONSIVE MICRORNAS; SHOOT APICAL MERISTEM; SMALL RNAS; EXPRESSION PATTERNS; MEDICAGO-TRUNCATULA; ARABIDOPSIS; REVEALS; IDENTIFICATION; GENES; TIME;

D O I：

10.1007/s00425-012-1634-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Hickory (Carya cathayensis Sarg.) is an economically important woody plant in China, but its long juvenile phase delays yield. MicroRNAs (miRNAs) are critical regulators of genes and important for normal plant development and physiology, including flower development. We used Solexa technology to sequence two small RNA libraries from two floral differentiation stages in hickory to identify miRNAs related to flower development. We identified 39 conserved miRNA sequences from 114 loci belonging to 23 families as well as two novel and ten potential novel miRNAs belonging to nine families. Moreover, 35 conserved miRNA*s and two novel miRNA*s were detected. Twenty miRNA sequences from 49 loci belonging to 11 families were differentially expressed; all were up-regulated at the later stage of flower development in hickory. Quantitative real-time PCR of 12 conserved miRNA sequences, five novel miRNA families, and two novel miRNA*s validated that all were expressed during hickory flower development, and the expression patterns were similar to those detected with Solexa sequencing. Finally, a total of 146 targets of the novel and conserved miRNAs were predicted. This study identified a diverse set of miRNAs that were closely related to hickory flower development and that could help in plant floral induction.

引用

页码：613 / 621

页数：9

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