Metabolome strategy against Edwardsiella tarda infection through glucose-enhanced metabolic modulation in tilapias

被引:48
作者
Peng, Bo [2 ]
Ma, Yan-mei [1 ]
Zhang, Jian-ying [2 ]
Li, Hui [1 ]
机构
[1] Sun Yat Sen Univ, Ctr Prote & Metabol, State Key Lab Biocontrol, MOE Key Lab Aquat Food Safety,Sch Life Sci, Guangzhou 510006, Guangdong, Peoples R China
[2] Univ Texas El Paso, Dept Biol Sci, El Paso, TX 79968 USA
基金
国家教育部博士点专项基金资助;
关键词
Edwardsiella tarda; Tilapias; Glucose; Metabolomics; Biomarker; BIOMARKERS; SURVIVAL;
D O I
10.1016/j.fsi.2015.06.004
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Edwardsiella tarda causes fish disease and great economic loss. However, metabolic strategy against the pathogen remains unexplored. In the present study, GC MS based metabolomics was used to investigate the metabolic profile from tilapias infected by sublethal dose of E. tarda. The metabolic differences between the dying group and survival group allow the identification of key pathways and crucial metabolites during infections. More importantly, those metabolites may modulate the survival-related metabolome to enhance the anti-infective ability. Our data showed that tilapias generated two different strategies, survival-metabolome and death-metabolome, to encounter EIB202 infection, leading to differential outputs of the survival and dying. Glucose was the most crucial biomarker, which was upregulated and downregulated in the survival and dying groups, respectively. Exogenous glucose by injection or oral administration enhanced hosts' ability against EIB202 infection and increased the chances of survival. These findings highlight that host mounts the metabolic strategy to cope with bacterial infection, from which crucial biomarkers may be identified to enhance the metabolic strategy. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:869 / 876
页数:8
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