Determining long-range chromatin interactions for selected genomic sites using 4C-seq technology: From fixation to computation

被引：171

作者：

Splinter, Erik ^{[1
]}

de Wit, Elzo ^{[1
]}

van de Werken, Harmen J. G. ^{[1
]}

Klous, Petra ^{[1
]}

de Laat, Wouter ^{[1
]}

机构：

[1] Univ Med Ctr Utrecht, Hubrecht Inst KNAW, NL-3584 CT Utrecht, Netherlands

来源：

METHODS | 2012年 / 58卷 / 03期

基金：

欧洲研究理事会;

关键词：

Nuclear organization; 4C Technology; HUMAN TRANSCRIPTOME MAP; CONFORMATION; DOMAINS; ORGANIZATION; INSIGHTS;

D O I：

10.1016/j.ymeth.2012.04.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromosome Conformation Capture (3C) and 3C-based technologies are constantly evolving in order to probe nuclear organization with higher depth and resolution. One such method is 4C-technology that allows the investigation of the nuclear environment of a locus of choice. The use of Illumina next generation sequencing as a detection platform for the analysis of 4C data has further improved the sensitivity and resolution of this method. Here we provide a step-by-step protocol for 4C-seq, describing the procedure from the initial template preparation until the final data analysis, interchanged with background information and considerations. (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：221 / 230

页数：10

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