Transglutaminase regulates immune-related genes in shrimp

被引：77

作者：

Fagutao, Fernand E. ^{[1
]}

Maningas, Mary Beth B. ^{[1
,2
,3
]}

Kondo, Hidehiro ^{[1
]}

Aoki, Takashi ^{[1
]}

Hirono, Ikuo ^{[1
]}

机构：

[1] Tokyo Univ Marine Sci & Technol, Grad Sch Marine Sci & Technol, Minato Ku, Tokyo 1088477, Japan

[2] Univ Santo Tomas, Dept Biol Sci, Coll Sci Mol Biol, Manila 1015, Philippines

[3] Univ Santo Tomas, Biotechnol Lab, Manila 1015, Philippines

来源：

FISH & SHELLFISH IMMUNOLOGY | 2012年 / 32卷 / 05期

基金：

日本学术振兴会;

关键词：

Transglutaminase; RNAi; Microarray analysis; Haemolymph coagulation; HEMOLYMPH CLOTTABLE PROTEINS; PLASMA CLOTTING PROTEIN; FRESH-WATER CRAYFISH; TIGER SHRIMP; MOLECULAR-CLONING; PENAEUS-MONODON; TISSUE LOCALIZATION; KURUMA PRAWN; EXPRESSION; COAGULATION;

D O I：

10.1016/j.fsi.2012.01.018

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Transglutaminase (TGase) is known to be involved in blood coagulation, a conserved defence mechanism among invertebrates. Gene silencing of TGase was previously shown to render shrimp susceptible to both bacterial and viral infections suggesting that TGase is an essential component of the shrimp immune system. Here, we examine the effects of the absence of TGase on the transcriptomic profile of kuruma shrimp by microarray analysis, focussing on genes that are involved in shrimp immunity. Total RNAs from shrimp haemocytes injected with dsRNA specific for TGase and control samples were isolated at 3 and 7 days p.i. and analyzed by microarray. Results revealed that TGase silencing affects the expression of genes in shrimp and caused significant down-regulation of the expressions of crustin and lysozyme. Furthermore, TGase-depleted samples were found to have lower haemocyte counts and higher total bacterial counts in their haemolymph. These results suggest that TGase is an important component of the shrimp immune response and is involved in the regulation of some immune-related genes particularly antimicrobial peptides. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：711 / 715

页数：5

共 44 条

[1] Molecular cloning, genomic organization and recombinant expression of a crustin-like antimicrobial peptide from black tiger shrimp Penaeus monodon [J].