Fluorescent molecularly imprinted nanogels for the detection of anticancer drugs in human plasma

被引：19
作者：
Pellizzoni, Elena [1 ,2 ]
Tommasini, Martina [1 ]
Marangon, Elena [3 ]
Rizzolio, Flavio [3 ]
Saito, Gabriele [4 ]
Benedetti, Fabio [1 ]
Toffoli, Giuseppe [3 ,5 ]
Resmini, Marina [4 ]
Berti, Federico [1 ]
机构：
[1] Univ Trieste, Dipartimento Sci Chim & Farmaceut, I-34127 Trieste, Italy
[2] Univ Trieste, Grad Sch Nanotechnol, I-34127 Trieste, Italy
[3] CRO Natl Canc Inst, Expt & Clin Pharmacol Div, Dept Translat Res, Aviano, Italy
[4] Queen Mary Univ London, SBCS, Dept Chem & Biochem, Mile End Rd, London E1 4NS, England
[5] IRCCS, Ctr Riferimento Oncol, Via Franco Gallini 2, I-33081 Aviano, Italy
来源：
BIOSENSORS & BIOELECTRONICS | 2016年 / 86卷
关键词：
Therapeutic drug monitoring; Anticancer drugs; Imprinted polymers; Human plasma; Fluorimetry; SELECTIVE EXTRACTION; CANCER-CHEMOTHERAPY; KINASE INHIBITORS; POLYMER; 5-FLUOROURACIL; SENSORS; MICROSPHERES; SPECTROSCOPY; TYROSINE; BINDING;
D O I：
10.1016/j.bios.2016.07.087
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Several fluorescent molecularly imprinted nanogels for the detection of the anticancer drug sunitinib were synthesized and characterized. A selection of functional monomers based on different aminoacids and coumarin allowed isolation of polymers with very good rebinding properties and sensitivities. The direct detection of sunitinib in human plasma was successfully demonstrated by fluorescence quenching of the coumarin-based nanogels. The plasma sample simply diluted in DMSO allowed the recovery of various amounts of sunitib, as determined by an averaged calibration curve. The LOD was 400 nM, with within-run variability <9%, day to day variability <5%, and good accuracy in the recovery of sunitinib from spiked samples. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：913 / 919
页数：7
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