Life sciences require the third dimension

被引：75

作者：

Keller, PJ ^{[1
]}

Pampaloni, F ^{[1
]}

Stelzer, EHK ^{[1
]}

机构：

[1] EMBL Heidelberg, Cell Biol & Biophys Unit, D-69117 Heidelberg, Germany

来源：

CURRENT OPINION IN CELL BIOLOGY | 2006年 / 18卷 / 01期

关键词：

D O I：

10.1016/j.ceb.2005.12.012

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Novel technologies are required for three-dimensional cell biology and biophysics. By three-dimensional we refer to experimental conditions that essentially try to avoid hard and flat surfaces and favour unconstrained sample dynamics. We believe that light-sheet-based microscopes are particularly well suited to studies of sensitive three-dimensional biological systems. The application of such instruments can be illustrated with examples from the biophysics of microtubule dynamics and three-dimensional cell cultures. Our experience leads us to suggest that three-dimensional approaches reveal new aspects of a system and enable experiments to be performed in a more physiological and hence clinically more relevant context.

引用

页码：117 / 124

页数：8

共 50 条

[1] Cell culture: Biology's new dimension [J].

Abbott, A .

NATURE, 2003, 424 (6951) :870-872

[2] CLIP-170/tubulin-curved oligomers coassemble at microtubule ends and promote rescues [J].

Arnal, I ;

Heichette, C ;

Diamantopoulos, GS ;

Chrétien, D .

CURRENT BIOLOGY, 2004, 14 (23) :2086-2095

[3] Putting tumours in context [J].

Bissell, MJ ;

Radisky, D .

NATURE REVIEWS CANCER, 2001, 1 (01) :46-54

[4] CLIP170-like tip1p spatially organizes microtubular dynamics in fission yeast [J].

Brunner, D ;

Nurse, P .

CELL, 2000, 102 (05) :695-704

[5]

CAMPBELL CJ, 1974, PHYSL OPTICS

[6] The fundamental properties of the fibroblast and the macrophage. II. The macrophage. [J].

Carrel, A ;

Ebeling, AH .

JOURNAL OF EXPERIMENTAL MEDICINE, 1926, 44 (03) :285-U2

[7]

CARREL A, 1910, J EXP MED, V13, P387

[8] Reconstruction of the centrosome cycle from cryoelectron micrographs [J].

Chrétien, D ;

Buendia, B ;

Fuller, SD ;

Karsenti, E .

JOURNAL OF STRUCTURAL BIOLOGY, 1997, 120 (02) :117-133

[9] Taking cell-matrix adhesions to the third dimension [J].

Cukierman, E ;

Pankov, R ;

Stevens, DR ;

Yamada, KM .

SCIENCE, 2001, 294 (5547) :1708-1712

[10] The role of apoptosis in creating and maintaining luminal space with normal and oncogene-expressing mammary acini [J].

Debnath, J ;

Mills, KR ;

Collins, NL ;

Reginato, MJ ;

Muthuswamy, SK ;

Brugge, JS .

CELL, 2002, 111 (01) :29-40

← 1 2 3 4 5 →