Comparative immunogenicity analysis of intradermal versus intramuscular immunization with a recombinant human adenovirus type 5 vaccine against Ebola virus

被引：16

作者：

Zhang, Zhe ^{[1
]}

Zhao, ZhengHao ^{[1
]}

Wang, Yudong ^{[1
]}

Wu, Shipo ^{[1
]}

Wang, Busen ^{[1
]}

Zhang, Jinlong ^{[1
]}

Song, Xiaohong ^{[1
]}

Chen, Yi ^{[1
]}

Lv, Peng ^{[1
]}

Hou, Lihua ^{[1
]}

机构：

[1] Beijing Inst Biotechnol, Lab Vaccine & Antibody Engn, Beijing, Peoples R China

来源：

FRONTIERS IN IMMUNOLOGY | 2022年 / 13卷

基金：

中国国家自然科学基金;

关键词：

immunogenicity; Ebola (EBOV); intradermal; intramuscular; Ad5-vector vaccine; T-CELL RESPONSES; DENDRITIC CELLS; VECTOR; DELIVERY; DECISION;

D O I：

10.3389/fimmu.2022.963049

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The proper route for vaccine delivery plays an important role in activating a robust immune response. Several viral vector-based vaccines against Ebola disease administered intramuscularly have been found to have excellent immunogenicity and protectiveness. In this study, we evaluated different vaccine routes for Ad5-EBOV delivery by comparing humoral and cellular responses, germinal center reactions, dendritic cell activation and antigen expression. Mice injected intramuscularly with the vaccine exhibited an advantage in antigen expression, leading to more robust germinal center and humoral responses, while intradermal injection recruited more migrating DCs and induced a more polyfunctional cellular response. Our study provides more data for future use of viral vector-based vaccines.

引用

页数：11

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